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Genome-wide chromatin interaction map for Trypanosoma cruzi

Trypanosomes are eukaryotic, unicellular parasites, such as Trypanosoma brucei, which causes sleeping sickness, and Trypanosoma cruzi, which causes Chagas disease. Genomes of these parasites comprise core regions and species-specific disruptive regions that encode multigene families of surface glyco...

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Autores principales: Díaz-Viraqué, Florencia, Chiribao, María Laura, Libisch, María Gabriela, Robello, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627812/
https://www.ncbi.nlm.nih.gov/pubmed/37828247
http://dx.doi.org/10.1038/s41564-023-01483-y
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author Díaz-Viraqué, Florencia
Chiribao, María Laura
Libisch, María Gabriela
Robello, Carlos
author_facet Díaz-Viraqué, Florencia
Chiribao, María Laura
Libisch, María Gabriela
Robello, Carlos
author_sort Díaz-Viraqué, Florencia
collection PubMed
description Trypanosomes are eukaryotic, unicellular parasites, such as Trypanosoma brucei, which causes sleeping sickness, and Trypanosoma cruzi, which causes Chagas disease. Genomes of these parasites comprise core regions and species-specific disruptive regions that encode multigene families of surface glycoproteins. Few transcriptional regulators have been identified in these parasites, and the role of spatial organization of the genome in gene expression is unclear. Here we mapped genome-wide chromatin interactions in T. cruzi using chromosome conformation capture (Hi-C), and we show that the core and disruptive regions form three-dimensional chromatin compartments named C and D. These chromatin compartments differ in levels of DNA methylation, nucleosome positioning and chromatin interactions, affecting genome expression dynamics. Our data reveal that the trypanosome genome is organized into chromatin-folding domains and transcription is affected by the local chromatin structure. We propose a model in which epigenetic mechanisms affect gene expression in trypanosomes.
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spelling pubmed-106278122023-11-08 Genome-wide chromatin interaction map for Trypanosoma cruzi Díaz-Viraqué, Florencia Chiribao, María Laura Libisch, María Gabriela Robello, Carlos Nat Microbiol Article Trypanosomes are eukaryotic, unicellular parasites, such as Trypanosoma brucei, which causes sleeping sickness, and Trypanosoma cruzi, which causes Chagas disease. Genomes of these parasites comprise core regions and species-specific disruptive regions that encode multigene families of surface glycoproteins. Few transcriptional regulators have been identified in these parasites, and the role of spatial organization of the genome in gene expression is unclear. Here we mapped genome-wide chromatin interactions in T. cruzi using chromosome conformation capture (Hi-C), and we show that the core and disruptive regions form three-dimensional chromatin compartments named C and D. These chromatin compartments differ in levels of DNA methylation, nucleosome positioning and chromatin interactions, affecting genome expression dynamics. Our data reveal that the trypanosome genome is organized into chromatin-folding domains and transcription is affected by the local chromatin structure. We propose a model in which epigenetic mechanisms affect gene expression in trypanosomes. Nature Publishing Group UK 2023-10-12 2023 /pmc/articles/PMC10627812/ /pubmed/37828247 http://dx.doi.org/10.1038/s41564-023-01483-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Díaz-Viraqué, Florencia
Chiribao, María Laura
Libisch, María Gabriela
Robello, Carlos
Genome-wide chromatin interaction map for Trypanosoma cruzi
title Genome-wide chromatin interaction map for Trypanosoma cruzi
title_full Genome-wide chromatin interaction map for Trypanosoma cruzi
title_fullStr Genome-wide chromatin interaction map for Trypanosoma cruzi
title_full_unstemmed Genome-wide chromatin interaction map for Trypanosoma cruzi
title_short Genome-wide chromatin interaction map for Trypanosoma cruzi
title_sort genome-wide chromatin interaction map for trypanosoma cruzi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627812/
https://www.ncbi.nlm.nih.gov/pubmed/37828247
http://dx.doi.org/10.1038/s41564-023-01483-y
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