Cargando…

Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters

The intracellular positioning of the centrosome, a major microtubule-organizing center, is important for cellular functions. One of the features of centrosome positioning is the spacing between centrosomes; however, the underlying mechanisms are not fully understood. To characterize the spacing acti...

Descripción completa

Detalles Bibliográficos
Autores principales: Fujii, Ken, Kondo, Tomo, Kimura, Akatsuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627822/
https://www.ncbi.nlm.nih.gov/pubmed/37931957
http://dx.doi.org/10.26508/lsa.202302427
_version_ 1785131611227947008
author Fujii, Ken
Kondo, Tomo
Kimura, Akatsuki
author_facet Fujii, Ken
Kondo, Tomo
Kimura, Akatsuki
author_sort Fujii, Ken
collection PubMed
description The intracellular positioning of the centrosome, a major microtubule-organizing center, is important for cellular functions. One of the features of centrosome positioning is the spacing between centrosomes; however, the underlying mechanisms are not fully understood. To characterize the spacing activity in Caenorhabditis elegans embryos, a genetic setup was developed to produce enucleated embryos. The centrosome was duplicated multiple times in the enucleated embryo, which enabled us to characterize the chromosome-independent spacing activity between sister and non-sister centrosome pairs. We found that the timely spacing depended on cytoplasmic dynein, and we propose a stoichiometric model of cortical and cytoplasmic pulling forces for the spacing between centrosomes. We also observed dynein-independent but non-muscle myosin II-dependent movement of centrosomes in the later cell cycle phase. The spacing mechanisms revealed in this study are expected to function between centrosomes in general, regardless of the presence of a chromosome/nucleus between them, including centrosome separation and spindle elongation.
format Online
Article
Text
id pubmed-10627822
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Life Science Alliance LLC
record_format MEDLINE/PubMed
spelling pubmed-106278222023-11-08 Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters Fujii, Ken Kondo, Tomo Kimura, Akatsuki Life Sci Alliance Research Articles The intracellular positioning of the centrosome, a major microtubule-organizing center, is important for cellular functions. One of the features of centrosome positioning is the spacing between centrosomes; however, the underlying mechanisms are not fully understood. To characterize the spacing activity in Caenorhabditis elegans embryos, a genetic setup was developed to produce enucleated embryos. The centrosome was duplicated multiple times in the enucleated embryo, which enabled us to characterize the chromosome-independent spacing activity between sister and non-sister centrosome pairs. We found that the timely spacing depended on cytoplasmic dynein, and we propose a stoichiometric model of cortical and cytoplasmic pulling forces for the spacing between centrosomes. We also observed dynein-independent but non-muscle myosin II-dependent movement of centrosomes in the later cell cycle phase. The spacing mechanisms revealed in this study are expected to function between centrosomes in general, regardless of the presence of a chromosome/nucleus between them, including centrosome separation and spindle elongation. Life Science Alliance LLC 2023-11-06 /pmc/articles/PMC10627822/ /pubmed/37931957 http://dx.doi.org/10.26508/lsa.202302427 Text en © 2023 Fujii et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Fujii, Ken
Kondo, Tomo
Kimura, Akatsuki
Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters
title Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters
title_full Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters
title_fullStr Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters
title_full_unstemmed Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters
title_short Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters
title_sort enucleation of the c. elegans embryo revealed dynein-dependent spacing between microtubule asters
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627822/
https://www.ncbi.nlm.nih.gov/pubmed/37931957
http://dx.doi.org/10.26508/lsa.202302427
work_keys_str_mv AT fujiiken enucleationofthecelegansembryorevealeddyneindependentspacingbetweenmicrotubuleasters
AT kondotomo enucleationofthecelegansembryorevealeddyneindependentspacingbetweenmicrotubuleasters
AT kimuraakatsuki enucleationofthecelegansembryorevealeddyneindependentspacingbetweenmicrotubuleasters