Synthetic oligonucleotides as quantitative PCR standards for quantifying microbial genes

Real-time quantitative PCR (qPCR) has been widely used to quantify gene copy numbers in microbial ecology. Despite its simplicity and straightforwardness, establishing qPCR assays is often impeded by the tedious process of producing qPCR standards by cloning the target DNA into plasmids. Here, we de...

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Detalles Bibliográficos
Autores principales: Han, Xingguo, Beck, Karin, Bürgmann, Helmut, Frey, Beat, Stierli, Beat, Frossard, Aline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627841/
https://www.ncbi.nlm.nih.gov/pubmed/37942081
http://dx.doi.org/10.3389/fmicb.2023.1279041

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627841/
https://www.ncbi.nlm.nih.gov/pubmed/37942081
http://dx.doi.org/10.3389/fmicb.2023.1279041

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