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Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia
OBJECTIVES: Accumulating evidence indicates a strong link between knee osteoarthritis (KOA) and sarcopenia. However, the mechanisms involved have not yet been elucidated. This study primarily aims to explore the molecular mechanisms that explain the connection between these 2 disorders. METHODS: The...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Osteoporosis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627980/ https://www.ncbi.nlm.nih.gov/pubmed/37941536 http://dx.doi.org/10.1016/j.afos.2023.08.005 |
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author | Yang, Jiyong Jiang, Tao Xu, Guangming Wang, Shuai Liu, Wengang |
author_facet | Yang, Jiyong Jiang, Tao Xu, Guangming Wang, Shuai Liu, Wengang |
author_sort | Yang, Jiyong |
collection | PubMed |
description | OBJECTIVES: Accumulating evidence indicates a strong link between knee osteoarthritis (KOA) and sarcopenia. However, the mechanisms involved have not yet been elucidated. This study primarily aims to explore the molecular mechanisms that explain the connection between these 2 disorders. METHODS: The gene expression profiles for KOA and sarcopenia were obtained from the Gene Expression Omnibus database, specifically from GSE55235, GSE169077, and GSE1408. Various bioinformatics techniques were employed to identify and analyze common differentially expressed genes (DEGs) across the 3 datasets. The techniques involved the analysis of Gene Ontology and pathways to enhance understanding, examining protein-protein interaction (PPI) networks, and identifying hub genes. In addition, we constructed the network of interactions between transcription factors (TFs) and genes, the co-regulatory network of TFs and miRNAs for hub genes, and predicted potential drugs. RESULTS: In total, 14 common DEGs were found between KOA and sarcopenia. Detailed information on biological processes and signaling pathways of common DEGs was obtained through enrichment analysis. After performing PPI network analysis, we discovered 4 hub genes (FOXO3, BCL6, CDKN1A, and CEBPB). Subsequently, we developed coregulatory networks for these hub genes involving TF-gene and TF-miRNA interactions. Finally, we identified 10 potential chemical compounds. CONCLUSIONS: By conducting bioinformatics analysis, our study has successfully identified common gene interaction networks between KOA and sarcopenia. The potential of these findings to offer revolutionary understanding into the common development of these 2 conditions could lead to the identification of valuable targets for therapy. |
format | Online Article Text |
id | pubmed-10627980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Korean Society of Osteoporosis |
record_format | MEDLINE/PubMed |
spelling | pubmed-106279802023-11-08 Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia Yang, Jiyong Jiang, Tao Xu, Guangming Wang, Shuai Liu, Wengang Osteoporos Sarcopenia Original Article OBJECTIVES: Accumulating evidence indicates a strong link between knee osteoarthritis (KOA) and sarcopenia. However, the mechanisms involved have not yet been elucidated. This study primarily aims to explore the molecular mechanisms that explain the connection between these 2 disorders. METHODS: The gene expression profiles for KOA and sarcopenia were obtained from the Gene Expression Omnibus database, specifically from GSE55235, GSE169077, and GSE1408. Various bioinformatics techniques were employed to identify and analyze common differentially expressed genes (DEGs) across the 3 datasets. The techniques involved the analysis of Gene Ontology and pathways to enhance understanding, examining protein-protein interaction (PPI) networks, and identifying hub genes. In addition, we constructed the network of interactions between transcription factors (TFs) and genes, the co-regulatory network of TFs and miRNAs for hub genes, and predicted potential drugs. RESULTS: In total, 14 common DEGs were found between KOA and sarcopenia. Detailed information on biological processes and signaling pathways of common DEGs was obtained through enrichment analysis. After performing PPI network analysis, we discovered 4 hub genes (FOXO3, BCL6, CDKN1A, and CEBPB). Subsequently, we developed coregulatory networks for these hub genes involving TF-gene and TF-miRNA interactions. Finally, we identified 10 potential chemical compounds. CONCLUSIONS: By conducting bioinformatics analysis, our study has successfully identified common gene interaction networks between KOA and sarcopenia. The potential of these findings to offer revolutionary understanding into the common development of these 2 conditions could lead to the identification of valuable targets for therapy. Korean Society of Osteoporosis 2023-09 2023-09-15 /pmc/articles/PMC10627980/ /pubmed/37941536 http://dx.doi.org/10.1016/j.afos.2023.08.005 Text en © 2023 The Korean Society of Osteoporosis. Publishing services by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Yang, Jiyong Jiang, Tao Xu, Guangming Wang, Shuai Liu, Wengang Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia |
title | Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia |
title_full | Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia |
title_fullStr | Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia |
title_full_unstemmed | Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia |
title_short | Exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia |
title_sort | exploring molecular mechanisms underlying the pathophysiological association between knee osteoarthritis and sarcopenia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627980/ https://www.ncbi.nlm.nih.gov/pubmed/37941536 http://dx.doi.org/10.1016/j.afos.2023.08.005 |
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