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The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice

Vitamin D deficiency is a global health problem and has been linked to defective spermatogenesis and male infertility. In this study, we aimed to investigate the main enzymes involved in the transsulfuration pathway of 1-carbon metabolism, and spermatogenesis function. Therefore, sixteen male C57 mi...

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Autores principales: Jamshidian-Ghalehsefidi, Narges, Rabiee, Farzaneh, Tavalaee, Marziyeh, Kiani, Shaghayegh, Pouriayevali, Farnaz, Razi, Mazdak, Dattilo, Maurizio, Nasr-Esfahani, Mohammad Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628119/
https://www.ncbi.nlm.nih.gov/pubmed/37932339
http://dx.doi.org/10.1038/s41598-023-45986-4
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author Jamshidian-Ghalehsefidi, Narges
Rabiee, Farzaneh
Tavalaee, Marziyeh
Kiani, Shaghayegh
Pouriayevali, Farnaz
Razi, Mazdak
Dattilo, Maurizio
Nasr-Esfahani, Mohammad Hossein
author_facet Jamshidian-Ghalehsefidi, Narges
Rabiee, Farzaneh
Tavalaee, Marziyeh
Kiani, Shaghayegh
Pouriayevali, Farnaz
Razi, Mazdak
Dattilo, Maurizio
Nasr-Esfahani, Mohammad Hossein
author_sort Jamshidian-Ghalehsefidi, Narges
collection PubMed
description Vitamin D deficiency is a global health problem and has been linked to defective spermatogenesis and male infertility. In this study, we aimed to investigate the main enzymes involved in the transsulfuration pathway of 1-carbon metabolism, and spermatogenesis function. Therefore, sixteen male C57 mice were addressed to a control (standard diet) or vitamin D deficient (VDD) diet for 14 weeks. The results show that compared to the standard diet, VDD increased final body weight and reduced sperm quality, caused damage to the testicular structure, and decreased the serum levels of testosterone. In addition, serum concentrations of homocysteine, vitamin B12, and sperm oxidative stress markers increased. In testicular tissues, the CBS and CSE protein levels were down-regulated whereas HO-1 was up-regulated at both mRNA and protein expression levels. Within a mice deprivation model, VDD deeply suppressed testosterone and impaired spermatogenesis with oxidative stress-mediated mechanisms. The effects of the deprivation appeared to be at least in part independent of genomic and receptor-mediated vitamin D actions and suggest a specific impairment of the alternative transsulfuration pathway.
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spelling pubmed-106281192023-11-08 The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice Jamshidian-Ghalehsefidi, Narges Rabiee, Farzaneh Tavalaee, Marziyeh Kiani, Shaghayegh Pouriayevali, Farnaz Razi, Mazdak Dattilo, Maurizio Nasr-Esfahani, Mohammad Hossein Sci Rep Article Vitamin D deficiency is a global health problem and has been linked to defective spermatogenesis and male infertility. In this study, we aimed to investigate the main enzymes involved in the transsulfuration pathway of 1-carbon metabolism, and spermatogenesis function. Therefore, sixteen male C57 mice were addressed to a control (standard diet) or vitamin D deficient (VDD) diet for 14 weeks. The results show that compared to the standard diet, VDD increased final body weight and reduced sperm quality, caused damage to the testicular structure, and decreased the serum levels of testosterone. In addition, serum concentrations of homocysteine, vitamin B12, and sperm oxidative stress markers increased. In testicular tissues, the CBS and CSE protein levels were down-regulated whereas HO-1 was up-regulated at both mRNA and protein expression levels. Within a mice deprivation model, VDD deeply suppressed testosterone and impaired spermatogenesis with oxidative stress-mediated mechanisms. The effects of the deprivation appeared to be at least in part independent of genomic and receptor-mediated vitamin D actions and suggest a specific impairment of the alternative transsulfuration pathway. Nature Publishing Group UK 2023-11-06 /pmc/articles/PMC10628119/ /pubmed/37932339 http://dx.doi.org/10.1038/s41598-023-45986-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jamshidian-Ghalehsefidi, Narges
Rabiee, Farzaneh
Tavalaee, Marziyeh
Kiani, Shaghayegh
Pouriayevali, Farnaz
Razi, Mazdak
Dattilo, Maurizio
Nasr-Esfahani, Mohammad Hossein
The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
title The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
title_full The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
title_fullStr The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
title_full_unstemmed The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
title_short The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
title_sort role of the transsulfuration pathway in spermatogenesis of vitamin d deficient mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628119/
https://www.ncbi.nlm.nih.gov/pubmed/37932339
http://dx.doi.org/10.1038/s41598-023-45986-4
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