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Differential early response of monocyte/macrophage subsets to intra-operative corticosteroid administration in lung transplantation

INTRODUCTION: Lung transplantation often results in primary and/or chronic dysfunctions that are related to early perioperative innate allo-responses where myeloid subsets play a major role. Corticosteroids are administered upon surgery as a standard-of-care but their action on the different myeloid...

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Detalles Bibliográficos
Autores principales: Glorion, Matthieu, Pascale, Florentina, Huriet, Maxime, Estephan, Jérôme, Gouin, Carla, Urien, Céline, Bourge, Mickael, Egidy, Giorgia, Richard, Christophe, Gelin, Valérie, De Wolf, Julien, Le Guen, Morgan, Magnan, Antoine, Roux, Antoine, Devillier, Philippe, Schwartz-Cornil, Isabelle, Sage, Edouard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628533/
https://www.ncbi.nlm.nih.gov/pubmed/37942330
http://dx.doi.org/10.3389/fimmu.2023.1281546
Descripción
Sumario:INTRODUCTION: Lung transplantation often results in primary and/or chronic dysfunctions that are related to early perioperative innate allo-responses where myeloid subsets play a major role. Corticosteroids are administered upon surgery as a standard-of-care but their action on the different myeloid cell subsets in that context is not known. METHODS: To address this issue, we used a cross-circulatory platform perfusing an extracorporeal lung coupled to cell mapping in the pig model, that enabled us to study the recruited cells in the allogeneic lung over 10 hours. RESULTS: Myeloid cells, i.e. granulocytes and monocytic cells including classical CD14(pos) and non-classical/intermediate CD16(pos) cells, were the dominantly recruited subsets, with the latter upregulating the membrane expression of MHC class II and CD80/86 molecules. Whereas corticosteroids did not reduce the different cell subset recruitment, they potently dampened the MHC class II and CD80/86 expression on monocytic cells and not on alveolar macrophages. Besides, corticosteroids induced a temporary and partial anti-inflammatory gene profile depending on cytokines and monocyte/macrophage subsets. DISCUSSION: This work documents the baseline effects of the standard-of-care corticosteroid treatment for early innate allo-responses. These insights will enable further optimization and improvement of lung transplantation outcomes.