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Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury

Adherens junctions between tubular epithelial cells are disrupted in renal ischemia/reperfusion (I/R) injury. Syndecan-1 (SDC-1) is involved in maintaining cell morphology. We aimed to study the role of SDC-1 shedding induced by renal I/R in the destruction of intracellular adherens junctions. We fo...

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Autores principales: Guo, Man, Shen, Daoqi, Su, Yiqi, Xu, Jiarui, Zhao, Shuan, Zhang, Weidong, Wang, Yaqiong, Jiang, Wuhua, Wang, Jialin, Geng, Xuemei, Ding, Xiaoqiang, Xu, Xialian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628745/
https://www.ncbi.nlm.nih.gov/pubmed/37942007
http://dx.doi.org/10.1016/j.isci.2023.108211
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author Guo, Man
Shen, Daoqi
Su, Yiqi
Xu, Jiarui
Zhao, Shuan
Zhang, Weidong
Wang, Yaqiong
Jiang, Wuhua
Wang, Jialin
Geng, Xuemei
Ding, Xiaoqiang
Xu, Xialian
author_facet Guo, Man
Shen, Daoqi
Su, Yiqi
Xu, Jiarui
Zhao, Shuan
Zhang, Weidong
Wang, Yaqiong
Jiang, Wuhua
Wang, Jialin
Geng, Xuemei
Ding, Xiaoqiang
Xu, Xialian
author_sort Guo, Man
collection PubMed
description Adherens junctions between tubular epithelial cells are disrupted in renal ischemia/reperfusion (I/R) injury. Syndecan-1 (SDC-1) is involved in maintaining cell morphology. We aimed to study the role of SDC-1 shedding induced by renal I/R in the destruction of intracellular adherens junctions. We found that SDC-1 shedding was increased while the expression of E-cadherin was decreased. This observation was accompanied by the activation of STAT3 in the kidneys. Inhibiting the shedding of SDC-1 induced by I/R could alleviate this effect. Mild renal I/R could induce more severe renal injury, lower E-cadherin expression, damaged cell junctions, and activated STAT3 in knockout mice with the tubule-specific deletion of SDC-1 mice. The results in vitro were consistent with those in vivo. Inhibiting the shedding of SDC-1 could alleviate the decreased expression of E-cadherin and damage of cell adherens junctions through inhibiting the activation of STAT3 during ischemic acute kidney injury.
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spelling pubmed-106287452023-11-08 Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury Guo, Man Shen, Daoqi Su, Yiqi Xu, Jiarui Zhao, Shuan Zhang, Weidong Wang, Yaqiong Jiang, Wuhua Wang, Jialin Geng, Xuemei Ding, Xiaoqiang Xu, Xialian iScience Article Adherens junctions between tubular epithelial cells are disrupted in renal ischemia/reperfusion (I/R) injury. Syndecan-1 (SDC-1) is involved in maintaining cell morphology. We aimed to study the role of SDC-1 shedding induced by renal I/R in the destruction of intracellular adherens junctions. We found that SDC-1 shedding was increased while the expression of E-cadherin was decreased. This observation was accompanied by the activation of STAT3 in the kidneys. Inhibiting the shedding of SDC-1 induced by I/R could alleviate this effect. Mild renal I/R could induce more severe renal injury, lower E-cadherin expression, damaged cell junctions, and activated STAT3 in knockout mice with the tubule-specific deletion of SDC-1 mice. The results in vitro were consistent with those in vivo. Inhibiting the shedding of SDC-1 could alleviate the decreased expression of E-cadherin and damage of cell adherens junctions through inhibiting the activation of STAT3 during ischemic acute kidney injury. Elsevier 2023-10-14 /pmc/articles/PMC10628745/ /pubmed/37942007 http://dx.doi.org/10.1016/j.isci.2023.108211 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Guo, Man
Shen, Daoqi
Su, Yiqi
Xu, Jiarui
Zhao, Shuan
Zhang, Weidong
Wang, Yaqiong
Jiang, Wuhua
Wang, Jialin
Geng, Xuemei
Ding, Xiaoqiang
Xu, Xialian
Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury
title Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury
title_full Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury
title_fullStr Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury
title_full_unstemmed Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury
title_short Syndecan-1 shedding destroys epithelial adherens junctions through STAT3 after renal ischemia/reperfusion injury
title_sort syndecan-1 shedding destroys epithelial adherens junctions through stat3 after renal ischemia/reperfusion injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628745/
https://www.ncbi.nlm.nih.gov/pubmed/37942007
http://dx.doi.org/10.1016/j.isci.2023.108211
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