A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells

Mucosal-associated invariant T (MAIT) cells are innate-like T cells responsible for mucosal immunity in the respiratory and intestinal tracts. Here we present a flow-cytometry-based assay to measure the cytolytic activity of murine MAIT cells and natural killer (NK) cells. We describe steps for diff...

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Detalles Bibliográficos
Autores principales: Sugimoto, Chie, Fujita, Hiroyoshi, Wakao, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628808/
http://dx.doi.org/10.1016/j.xpro.2023.102620
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author Sugimoto, Chie
Fujita, Hiroyoshi
Wakao, Hiroshi
author_facet Sugimoto, Chie
Fujita, Hiroyoshi
Wakao, Hiroshi
author_sort Sugimoto, Chie
collection PubMed
description Mucosal-associated invariant T (MAIT) cells are innate-like T cells responsible for mucosal immunity in the respiratory and intestinal tracts. Here we present a flow-cytometry-based assay to measure the cytolytic activity of murine MAIT cells and natural killer (NK) cells. We describe steps for differentiating MAIT-like cells from the induced pluripotent stem cells prepared from MAIT cells (reMAIT cells), NK cell isolation, co-culture with target tumor cells, and staining to distinguish dead cells from live cells. For complete details on the use and execution of this protocol, please refer to Sugimoto et al. (2022).(1)
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spelling pubmed-106288082023-11-08 A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells Sugimoto, Chie Fujita, Hiroyoshi Wakao, Hiroshi STAR Protoc Protocol Mucosal-associated invariant T (MAIT) cells are innate-like T cells responsible for mucosal immunity in the respiratory and intestinal tracts. Here we present a flow-cytometry-based assay to measure the cytolytic activity of murine MAIT cells and natural killer (NK) cells. We describe steps for differentiating MAIT-like cells from the induced pluripotent stem cells prepared from MAIT cells (reMAIT cells), NK cell isolation, co-culture with target tumor cells, and staining to distinguish dead cells from live cells. For complete details on the use and execution of this protocol, please refer to Sugimoto et al. (2022).(1) Elsevier 2023-10-27 /pmc/articles/PMC10628808/ http://dx.doi.org/10.1016/j.xpro.2023.102620 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Sugimoto, Chie
Fujita, Hiroyoshi
Wakao, Hiroshi
A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells
title A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells
title_full A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells
title_fullStr A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells
title_full_unstemmed A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells
title_short A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cells
title_sort flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse mait cells and natural killer cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628808/
http://dx.doi.org/10.1016/j.xpro.2023.102620
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