Protocol for screening cellular outputs activated by optogenetically controlled temporal PI3K signaling activation patterns

PI3K signaling elicits distinct outputs in response to different patterns of extracellular stimulation. Here, we present a protocol for screening cellular outputs activated by optogenetically controlled temporal PI3K signaling activation patterns in 96-well plates. We describe steps for establishing...

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Detalles Bibliográficos
Autores principales: Ueda, Yoshibumi, Matsushita, Shohei, Suzuki, Mitsugu, Ozawa, Takeaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628809/
http://dx.doi.org/10.1016/j.xpro.2023.102622

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