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Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder

INTRODUCTION: Environmental and genetic factors contribute to the increased risk for neurodevelopmental disorders, including deficits in the development of social communication. In the mouse, ultrasonic vocalizations emitted by the pup stimulate maternal retrieval and potentiate maternal care. There...

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Autores principales: Gal, Ayelet, Raykin, Eynav, Giladi, Shaked, Lederman, Dror, Kofman, Ora, Golan, Hava M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629105/
https://www.ncbi.nlm.nih.gov/pubmed/37942134
http://dx.doi.org/10.3389/fnins.2023.1274039
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author Gal, Ayelet
Raykin, Eynav
Giladi, Shaked
Lederman, Dror
Kofman, Ora
Golan, Hava M.
author_facet Gal, Ayelet
Raykin, Eynav
Giladi, Shaked
Lederman, Dror
Kofman, Ora
Golan, Hava M.
author_sort Gal, Ayelet
collection PubMed
description INTRODUCTION: Environmental and genetic factors contribute to the increased risk for neurodevelopmental disorders, including deficits in the development of social communication. In the mouse, ultrasonic vocalizations emitted by the pup stimulate maternal retrieval and potentiate maternal care. Therefore, isolation induced ultrasonic vocalization emitted by pups provides a means to evaluate deficits in communication during early development, before other ways of communication are apparent. Previous studies in our labs showed that gestational exposure to the pesticide chlorpyrifos (CPF) and the Methylenetetrahydrofolate (Mthfr)-knock-out mice are associated with impaired social preference, restricted or repetitive behavior and altered spectral properties of pups’ ultrasonic vocalization. In this study, we explore the temporal dynamics of pups’ vocalization in these Autism spectrum disorder (ASD) models. METHODS: We utilized the maternal potentiation protocol and analyzed the time course of pup vocalizations following isolation from the nest. Two models of ASD were studied: gestational exposure to the pesticide CPF and the Mthfr-knock-out mice. RESULTS: Vocalization emitted by pups of both ASD models were dynamically modified in quantity and spectral structure within each session and between the two isolation sessions. The first isolation session was characterized by a buildup of call quantity and significant effects of USV spectral structure variables, and the second isolation session was characterized by enhanced calls and vocalization time, but minute effect on USV properties. Moreover, in both models we described an increased usage of harmonic calls with time during the isolation sessions. DISCUSSION: Communication between two or more individuals requires an interplay between the two sides and depends on the response and the time since the stimulus. As such, the presence of dynamic changes in vocalization structure in the control pups, and the alteration observed in the pups of the ASD models, suggest impaired regulation of vocalization associated with the environmental and genetic factors. Last, we propose that temporal dynamics of ultrasonic vocalization communication should be considered in future analysis in rodent models of ASD to maximize the sensitivity of the study of vocalizations.
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spelling pubmed-106291052023-11-08 Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder Gal, Ayelet Raykin, Eynav Giladi, Shaked Lederman, Dror Kofman, Ora Golan, Hava M. Front Neurosci Neuroscience INTRODUCTION: Environmental and genetic factors contribute to the increased risk for neurodevelopmental disorders, including deficits in the development of social communication. In the mouse, ultrasonic vocalizations emitted by the pup stimulate maternal retrieval and potentiate maternal care. Therefore, isolation induced ultrasonic vocalization emitted by pups provides a means to evaluate deficits in communication during early development, before other ways of communication are apparent. Previous studies in our labs showed that gestational exposure to the pesticide chlorpyrifos (CPF) and the Methylenetetrahydrofolate (Mthfr)-knock-out mice are associated with impaired social preference, restricted or repetitive behavior and altered spectral properties of pups’ ultrasonic vocalization. In this study, we explore the temporal dynamics of pups’ vocalization in these Autism spectrum disorder (ASD) models. METHODS: We utilized the maternal potentiation protocol and analyzed the time course of pup vocalizations following isolation from the nest. Two models of ASD were studied: gestational exposure to the pesticide CPF and the Mthfr-knock-out mice. RESULTS: Vocalization emitted by pups of both ASD models were dynamically modified in quantity and spectral structure within each session and between the two isolation sessions. The first isolation session was characterized by a buildup of call quantity and significant effects of USV spectral structure variables, and the second isolation session was characterized by enhanced calls and vocalization time, but minute effect on USV properties. Moreover, in both models we described an increased usage of harmonic calls with time during the isolation sessions. DISCUSSION: Communication between two or more individuals requires an interplay between the two sides and depends on the response and the time since the stimulus. As such, the presence of dynamic changes in vocalization structure in the control pups, and the alteration observed in the pups of the ASD models, suggest impaired regulation of vocalization associated with the environmental and genetic factors. Last, we propose that temporal dynamics of ultrasonic vocalization communication should be considered in future analysis in rodent models of ASD to maximize the sensitivity of the study of vocalizations. Frontiers Media S.A. 2023-10-23 /pmc/articles/PMC10629105/ /pubmed/37942134 http://dx.doi.org/10.3389/fnins.2023.1274039 Text en Copyright © 2023 Gal, Raykin, Giladi, Lederman, Kofman and Golan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Gal, Ayelet
Raykin, Eynav
Giladi, Shaked
Lederman, Dror
Kofman, Ora
Golan, Hava M.
Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder
title Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder
title_full Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder
title_fullStr Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder
title_full_unstemmed Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder
title_short Temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder
title_sort temporal dynamics of isolation calls emitted by pups in environmental and genetic mouse models of autism spectrum disorder
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629105/
https://www.ncbi.nlm.nih.gov/pubmed/37942134
http://dx.doi.org/10.3389/fnins.2023.1274039
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