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Active Site Loop Engineering Abolishes Water Capture in Hydroxylating Sesquiterpene Synthases

[Image: see text] Terpene synthases (TS) catalyze complex reactions to produce a diverse array of terpene skeletons from linear isoprenyl diphosphates. Patchoulol synthase (PTS) from Pogostemon cablin converts farnesyl diphosphate into patchoulol. Using simulation-guided engineering, we obtained PTS...

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Detalles Bibliográficos
Autores principales:	Srivastava, Prabhakar L., Johns, Sam T., Walters, Rebecca, Miller, David J., Van der Kamp, Marc W., Allemann, Rudolf K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629212/ https://www.ncbi.nlm.nih.gov/pubmed/37942265 http://dx.doi.org/10.1021/acscatal.3c03920

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