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Medium Depth Influences O(2) Availability and Metabolism in Human RPE Cultures

PURPOSE: Retinal pigment epithelium (RPE) oxidative metabolism is critical for normal retinal function and is often studied in cell culture systems. Here, we show that conventional culture media volumes dramatically impact O(2) availability, limiting oxidative metabolism. We suggest optimal conditio...

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Detalles Bibliográficos
Autores principales:	Hass, Daniel T., Zhang, Qitao, Autterson, Gillian A., Bryan, Richard A., Hurley, James B., Miller, Jason M. L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Association for Research in Vision and Ophthalmology 2023
Materias:	Retinal Cell Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629522/ https://www.ncbi.nlm.nih.gov/pubmed/37922158 http://dx.doi.org/10.1167/iovs.64.14.4

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