Cargando…

Medium Depth Influences O(2) Availability and Metabolism in Human RPE Cultures

PURPOSE: Retinal pigment epithelium (RPE) oxidative metabolism is critical for normal retinal function and is often studied in cell culture systems. Here, we show that conventional culture media volumes dramatically impact O(2) availability, limiting oxidative metabolism. We suggest optimal conditio...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hass, Daniel T., Zhang, Qitao, Autterson, Gillian A., Bryan, Richard A., Hurley, James B., Miller, Jason M. L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Association for Research in Vision and Ophthalmology 2023
Materias:	Retinal Cell Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629522/ https://www.ncbi.nlm.nih.gov/pubmed/37922158 http://dx.doi.org/10.1167/iovs.64.14.4

Ejemplares similares

Highly Differentiated Human Fetal RPE Cultures Are Resistant to the Accumulation and Toxicity of Lipofuscin-Like Material
por: Zhang, Qitao, et al.
Publicado: (2019)

Complement-Mediated Regulation of Apolipoprotein E in Cultured Human RPE Cells
por: Yang, Ping, et al.
Publicado: (2017)

Albumen Transport to Bruch's Membrane and RPE by Choriocapillaris Caveolae
por: Nakanishi, Masataka, et al.
Publicado: (2016)

Risuteganib Protects against Hydroquinone–induced Injury in Human RPE Cells
por: Yang, Ping, et al.
Publicado: (2020)

PGC-1α Induces Human RPE Oxidative Metabolism and Antioxidant Capacity
por: Iacovelli, Jared, et al.
Publicado: (2016)

Characterization of Human iPSC-RPE on a Prosthetic Bruch's Membrane Manufactured From Silk Fibroin
por: Galloway, Chad A., et al.
Publicado: (2018)

Deletion of Efemp1 Is Protective Against the Development of Sub-RPE Deposits in Mouse Eyes
por: Stanton, James B., et al.
Publicado: (2017)

Retina Organoid Transplants Develop Photoreceptors and Improve Visual Function in RCS Rats With RPE Dysfunction
por: Lin, Bin, et al.
Publicado: (2020)

DHA Shortage Causes the Early Degeneration of Photoreceptors and RPE in Mice With Peroxisomal β-Oxidation Deficiency
por: Swinkels, Daniëlle, et al.
Publicado: (2023)

Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
por: Altera, Annalisa, et al.
Publicado: (2021)

The Mitochondrial-Derived Peptide Humanin Protects RPE Cells From Oxidative Stress, Senescence, and Mitochondrial Dysfunction
por: Sreekumar, Parameswaran G., et al.
Publicado: (2016)

Conditional, Genetically Encoded, Small Molecule–Regulated Inhibition of NFκB Signaling in RPE Cells
por: Vu, Khiem T., et al.
Publicado: (2017)

Spatiotemporal Coordination of RPE Cell Quality by Extracellular Vesicle miR-494-3p Via Competitive Interplays With SIRT3 or PTEN
por: Hamuro, Junji, et al.
Publicado: (2023)

Subretinal Pigment Epithelial Deposition of Drusen Components Including Hydroxyapatite in a Primary Cell Culture Model
por: Pilgrim, Matthew G., et al.
Publicado: (2017)

A Targeted Inhibitor of the Alternative Complement Pathway Accelerates Recovery From Smoke-Induced Ocular Injury
por: Woodell, Alex, et al.
Publicado: (2016)

Anatomical and Gene Expression Changes in the Retinal Pigmented Epithelium Atrophy 1 (rpea1) Mouse: A Potential Model of Serous Retinal Detachment
por: Luna, Gabriel, et al.
Publicado: (2016)

Analysis of Adult Neural Retina Extracellular Vesicle Release, RNA Transport and Proteomic Cargo
por: Mighty, Jason, et al.
Publicado: (2020)

Potential of Small Molecule–Mediated Reprogramming of Rod Photoreceptors to Treat Retinitis Pigmentosa
por: Nakamura, Paul A., et al.
Publicado: (2016)

Geographic Atrophy: Confocal Scanning Laser Ophthalmoscopy, Histology, and Inflammation in the Region of Expanding Lesions
por: Bonilha, Vera L., et al.
Publicado: (2020)

Retinal Glial and Choroidal Vascular Pathology in Donors Clinically Diagnosed With Stargardt Disease
por: Edwards, Malia M., et al.
Publicado: (2020)

N-Acetylcysteine Amide Protects Against Oxidative Stress–Induced Microparticle Release From Human Retinal Pigment Epithelial Cells
por: Carver, Kyle A., et al.
Publicado: (2016)

The Role of DNA Methylation in the Metabolic Memory Phenomenon Associated With the Continued Progression of Diabetic Retinopathy
por: Mishra, Manish, et al.
Publicado: (2016)

NADPH Oxidase Contributes to Photoreceptor Degeneration in Constitutively Active RAC1 Mice
por: Song, Hongman, et al.
Publicado: (2016)

Subretinal Glial Membranes in Eyes With Geographic Atrophy
por: Edwards, Malia M., et al.
Publicado: (2017)

Adenosine Deaminase-2–Induced Hyperpermeability in Human Retinal Vascular Endothelial Cells Is Suppressed by MicroRNA-146b-3p
por: Samra, Yara A., et al.
Publicado: (2017)

Epigenetic Modifications Compromise Mitochondrial DNA Quality Control in the Development of Diabetic Retinopathy
por: Mohammad, Ghulam, et al.
Publicado: (2019)

Hydrogen Sulfide: A Potential Therapeutic Target in the Development of Diabetic Retinopathy
por: Mohammad, Ghulam, et al.
Publicado: (2020)

Crosstalk Between Histone and DNA Methylation in Regulation of Retinal Matrix Metalloproteinase-9 in Diabetes
por: Duraisamy, Arul J., et al.
Publicado: (2017)

Peripheral Blood Mitochondrial DNA Damage as a Potential Noninvasive Biomarker of Diabetic Retinopathy
por: Mishra, Manish, et al.
Publicado: (2016)

Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses
por: Schaefer, Kellie A., et al.
Publicado: (2016)

Epigenetics and Regulation of Oxidative Stress in Diabetic Retinopathy
por: Duraisamy, Arul J., et al.
Publicado: (2018)

Knockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1
por: Liu, Fanfei, et al.
Publicado: (2021)

Diabetes Modulates Iodothyronine Deiodinase 2 Expression in the Mouse Retina: A Role for Thyroid Hormone in the Pathogenesis of Diabetic Retinopathy
por: Bapputty, Reena, et al.
Publicado: (2023)

Rapid, Dynamic Activation of Müller Glial Stem Cell Responses in Zebrafish
por: Sifuentes, Christopher J., et al.
Publicado: (2016)

Oxidized Lipoprotein Uptake Through the CD36 Receptor Activates the NLRP3 Inflammasome in Human Retinal Pigment Epithelial Cells
por: Gnanaguru, Gopalan, et al.
Publicado: (2016)

The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina
por: Groman-Lupa, Sergio, et al.
Publicado: (2017)

Novel Animal Model of Crumbs-Dependent Progressive Retinal Degeneration That Targets Specific Cone Subtypes
por: Fu, Jinling, et al.
Publicado: (2018)

Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina
por: Park, Ko Uoon, et al.
Publicado: (2017)

Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3
por: Esposito, Nicholas J., et al.
Publicado: (2021)

Signaling Through the Erythropoietin Receptor Affects Angiogenesis in Retinovascular Disease
por: Bretz, Colin A., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_a75virkbj9bq80drfbndpl0tgv