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Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly

Protein–RNA complexes exist in many forms within the cell, from stable machines such as the ribosome to transient assemblies like the spliceosome. All protein–RNA assemblies rely on spatially and temporally coordinated interactions between specific proteins and RNAs to achieve a functional form. RNA...

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Autores principales: Höfler, Simone, Duss, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629537/
https://www.ncbi.nlm.nih.gov/pubmed/37935465
http://dx.doi.org/10.26508/lsa.202302240
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author Höfler, Simone
Duss, Olivier
author_facet Höfler, Simone
Duss, Olivier
author_sort Höfler, Simone
collection PubMed
description Protein–RNA complexes exist in many forms within the cell, from stable machines such as the ribosome to transient assemblies like the spliceosome. All protein–RNA assemblies rely on spatially and temporally coordinated interactions between specific proteins and RNAs to achieve a functional form. RNA folding and structure are often critical for successful protein binding and protein–RNA complex formation. RNA modifications change the chemical nature of a given RNA and often alter its folding kinetics. Both these alterations can affect how and if proteins or other RNAs can interact with the modified RNA and assemble into complexes. N(6)-methyladenosine (m(6)A) is the most common base modification on mRNAs and regulatory noncoding RNAs and has been shown to impact RNA structure and directly modulate protein–RNA interactions. In this review, focusing on the mechanisms and available quantitative information, we discuss first how the METTL3/14 m(6)A writer complex is specifically targeted to RNA assisted by protein–RNA and other interactions to enable site-specific and co-transcriptional RNA modification and, once introduced, how the m(6)A modification affects RNA folding and protein–RNA interactions.
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spelling pubmed-106295372023-11-08 Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly Höfler, Simone Duss, Olivier Life Sci Alliance Reviews Protein–RNA complexes exist in many forms within the cell, from stable machines such as the ribosome to transient assemblies like the spliceosome. All protein–RNA assemblies rely on spatially and temporally coordinated interactions between specific proteins and RNAs to achieve a functional form. RNA folding and structure are often critical for successful protein binding and protein–RNA complex formation. RNA modifications change the chemical nature of a given RNA and often alter its folding kinetics. Both these alterations can affect how and if proteins or other RNAs can interact with the modified RNA and assemble into complexes. N(6)-methyladenosine (m(6)A) is the most common base modification on mRNAs and regulatory noncoding RNAs and has been shown to impact RNA structure and directly modulate protein–RNA interactions. In this review, focusing on the mechanisms and available quantitative information, we discuss first how the METTL3/14 m(6)A writer complex is specifically targeted to RNA assisted by protein–RNA and other interactions to enable site-specific and co-transcriptional RNA modification and, once introduced, how the m(6)A modification affects RNA folding and protein–RNA interactions. Life Science Alliance LLC 2023-11-07 /pmc/articles/PMC10629537/ /pubmed/37935465 http://dx.doi.org/10.26508/lsa.202302240 Text en © 2023 Höfler and Duss https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Reviews
Höfler, Simone
Duss, Olivier
Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly
title Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly
title_full Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly
title_fullStr Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly
title_full_unstemmed Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly
title_short Interconnections between m(6)A RNA modification, RNA structure, and protein–RNA complex assembly
title_sort interconnections between m(6)a rna modification, rna structure, and protein–rna complex assembly
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629537/
https://www.ncbi.nlm.nih.gov/pubmed/37935465
http://dx.doi.org/10.26508/lsa.202302240
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