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Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells

During human forebrain development, neural progenitor cells (NPCs) in the ventricular zone (VZ) undergo asymmetric cell divisions to produce a self-renewed progenitor cell, maintaining the potential to go through additional rounds of cell divisions, and differentiating daughter cells, populating the...

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Autores principales: Royall, Lars N, Machado, Diana, Jessberger, Sebastian, Denoth-Lippuner, Annina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629821/
https://www.ncbi.nlm.nih.gov/pubmed/37882444
http://dx.doi.org/10.7554/eLife.83157
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author Royall, Lars N
Machado, Diana
Jessberger, Sebastian
Denoth-Lippuner, Annina
author_facet Royall, Lars N
Machado, Diana
Jessberger, Sebastian
Denoth-Lippuner, Annina
author_sort Royall, Lars N
collection PubMed
description During human forebrain development, neural progenitor cells (NPCs) in the ventricular zone (VZ) undergo asymmetric cell divisions to produce a self-renewed progenitor cell, maintaining the potential to go through additional rounds of cell divisions, and differentiating daughter cells, populating the developing cortex. Previous work in the embryonic rodent brain suggested that the preferential inheritance of the pre-existing (older) centrosome to the self-renewed progenitor cell is required to maintain stem cell properties, ensuring proper neurogenesis. If asymmetric segregation of centrosomes occurs in NPCs of the developing human brain, which depends on unique molecular regulators and species-specific cellular composition, remains unknown. Using a novel, recombination-induced tag exchange-based genetic tool to birthdate and track the segregation of centrosomes over multiple cell divisions in human embryonic stem cell-derived regionalised forebrain organoids, we show the preferential inheritance of the older mother centrosome towards self-renewed NPCs. Aberration of asymmetric segregation of centrosomes by genetic manipulation of the centrosomal, microtubule-associated protein Ninein alters fate decisions of NPCs and their maintenance in the VZ of human cortical organoids. Thus, the data described here use a novel genetic approach to birthdate centrosomes in human cells and identify asymmetric inheritance of centrosomes as a mechanism to maintain self-renewal properties and to ensure proper neurogenesis in human NPCs.
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spelling pubmed-106298212023-11-08 Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells Royall, Lars N Machado, Diana Jessberger, Sebastian Denoth-Lippuner, Annina eLife Developmental Biology During human forebrain development, neural progenitor cells (NPCs) in the ventricular zone (VZ) undergo asymmetric cell divisions to produce a self-renewed progenitor cell, maintaining the potential to go through additional rounds of cell divisions, and differentiating daughter cells, populating the developing cortex. Previous work in the embryonic rodent brain suggested that the preferential inheritance of the pre-existing (older) centrosome to the self-renewed progenitor cell is required to maintain stem cell properties, ensuring proper neurogenesis. If asymmetric segregation of centrosomes occurs in NPCs of the developing human brain, which depends on unique molecular regulators and species-specific cellular composition, remains unknown. Using a novel, recombination-induced tag exchange-based genetic tool to birthdate and track the segregation of centrosomes over multiple cell divisions in human embryonic stem cell-derived regionalised forebrain organoids, we show the preferential inheritance of the older mother centrosome towards self-renewed NPCs. Aberration of asymmetric segregation of centrosomes by genetic manipulation of the centrosomal, microtubule-associated protein Ninein alters fate decisions of NPCs and their maintenance in the VZ of human cortical organoids. Thus, the data described here use a novel genetic approach to birthdate centrosomes in human cells and identify asymmetric inheritance of centrosomes as a mechanism to maintain self-renewal properties and to ensure proper neurogenesis in human NPCs. eLife Sciences Publications, Ltd 2023-10-26 /pmc/articles/PMC10629821/ /pubmed/37882444 http://dx.doi.org/10.7554/eLife.83157 Text en © 2023, Royall et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Royall, Lars N
Machado, Diana
Jessberger, Sebastian
Denoth-Lippuner, Annina
Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
title Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
title_full Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
title_fullStr Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
title_full_unstemmed Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
title_short Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
title_sort asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629821/
https://www.ncbi.nlm.nih.gov/pubmed/37882444
http://dx.doi.org/10.7554/eLife.83157
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