RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System

Inducible gene regulation methods are indispensable in diverse biological applications, yet many of them have severe limitations in their applicability. These include inducer toxicity, a limited variety of organisms the given system can be used in, and side effects of the induction method. In this s...

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Detalles Bibliográficos
Autores principales: Meinzinger, Anne, Zsigmond, Áron, Horváth, Péter, Kellenberger, Alexandra, Paréj, Katalin, Tallone, Tiziano, Flachner, Beáta, Cserhalmi, Marcell, Lőrincz, Zsolt, Cseh, Sándor, Shmerling, Doron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630016/
https://www.ncbi.nlm.nih.gov/pubmed/37941807
http://dx.doi.org/10.1155/2023/7121512
Descripción
Sumario:Inducible gene regulation methods are indispensable in diverse biological applications, yet many of them have severe limitations in their applicability. These include inducer toxicity, a limited variety of organisms the given system can be used in, and side effects of the induction method. In this study, a novel inducible system, the RuX system, was created using a mutant ligand-binding domain of the glucocorticoid receptor (CS1/CD), used together with various genetic elements such as the Gal4 DNA-binding domain or Cre recombinase. The RuX system is shown to be capable of over 1000-fold inducibility, has flexible applications, and is offered for use in cell cultures.

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