Cargando…

Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells

Omentin-1 (OMNT1) is an adipokine involved in the regulation of energy metabolism, insulin sensitivity, and reproduction. The present study was the first to investigate the plasma levels and expression of OMNT1 in the anterior pituitary (AP) gland on days 2–3, 10–12, 14–16, and 17–19 of the estrous...

Descripción completa

Detalles Bibliográficos
Autores principales: Respekta, Natalia, Pich, Karolina, Mlyczyńska, Ewa, Dobrzyń, Kamil, Ramé, Christelle, Kamiński, Tadeusz, Smolińska, Nina, Dupont, Joëlle, Rak, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630491/
https://www.ncbi.nlm.nih.gov/pubmed/37935840
http://dx.doi.org/10.1038/s41598-023-46742-4
_version_ 1785132160862126080
author Respekta, Natalia
Pich, Karolina
Mlyczyńska, Ewa
Dobrzyń, Kamil
Ramé, Christelle
Kamiński, Tadeusz
Smolińska, Nina
Dupont, Joëlle
Rak, Agnieszka
author_facet Respekta, Natalia
Pich, Karolina
Mlyczyńska, Ewa
Dobrzyń, Kamil
Ramé, Christelle
Kamiński, Tadeusz
Smolińska, Nina
Dupont, Joëlle
Rak, Agnieszka
author_sort Respekta, Natalia
collection PubMed
description Omentin-1 (OMNT1) is an adipokine involved in the regulation of energy metabolism, insulin sensitivity, and reproduction. The present study was the first to investigate the plasma levels and expression of OMNT1 in the anterior pituitary (AP) gland on days 2–3, 10–12, 14–16, and 17–19 of the estrous cycle of normal-weight Large White (LW) and fat Meishan (MS) pigs. Next, we determined the effect of GnRH, LH, and FSH on the OMNT1 levels in cultured AP cells. The gene and protein expression of OMNT1 in AP fluctuated during the estrous cycle, with a higher expression in MS than in LW (except on days 10–12). However, plasma levels of OMNT1 were higher in LW than in MS. OMNT1 was localized in somatotrophs, lactotrophs, thyrotrophs, and gonadotrophs. In LW pituitary cells, GnRH and gonadotropins stimulated OMNT1 protein expression (except FSH on days 14–16) and had no effect on OMNT1 levels in the culture medium. In MS pituitary cells, we observed that GnRH and LH increased while FSH decreased OMNT1 protein expression. These findings showed OMNT1 expression and regulation in the porcine AP and suggested that OMNT1 could be a new player modifying the pituitary functions.
format Online
Article
Text
id pubmed-10630491
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106304912023-11-07 Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells Respekta, Natalia Pich, Karolina Mlyczyńska, Ewa Dobrzyń, Kamil Ramé, Christelle Kamiński, Tadeusz Smolińska, Nina Dupont, Joëlle Rak, Agnieszka Sci Rep Article Omentin-1 (OMNT1) is an adipokine involved in the regulation of energy metabolism, insulin sensitivity, and reproduction. The present study was the first to investigate the plasma levels and expression of OMNT1 in the anterior pituitary (AP) gland on days 2–3, 10–12, 14–16, and 17–19 of the estrous cycle of normal-weight Large White (LW) and fat Meishan (MS) pigs. Next, we determined the effect of GnRH, LH, and FSH on the OMNT1 levels in cultured AP cells. The gene and protein expression of OMNT1 in AP fluctuated during the estrous cycle, with a higher expression in MS than in LW (except on days 10–12). However, plasma levels of OMNT1 were higher in LW than in MS. OMNT1 was localized in somatotrophs, lactotrophs, thyrotrophs, and gonadotrophs. In LW pituitary cells, GnRH and gonadotropins stimulated OMNT1 protein expression (except FSH on days 14–16) and had no effect on OMNT1 levels in the culture medium. In MS pituitary cells, we observed that GnRH and LH increased while FSH decreased OMNT1 protein expression. These findings showed OMNT1 expression and regulation in the porcine AP and suggested that OMNT1 could be a new player modifying the pituitary functions. Nature Publishing Group UK 2023-11-07 /pmc/articles/PMC10630491/ /pubmed/37935840 http://dx.doi.org/10.1038/s41598-023-46742-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Respekta, Natalia
Pich, Karolina
Mlyczyńska, Ewa
Dobrzyń, Kamil
Ramé, Christelle
Kamiński, Tadeusz
Smolińska, Nina
Dupont, Joëlle
Rak, Agnieszka
Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
title Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
title_full Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
title_fullStr Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
title_full_unstemmed Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
title_short Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
title_sort plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630491/
https://www.ncbi.nlm.nih.gov/pubmed/37935840
http://dx.doi.org/10.1038/s41598-023-46742-4
work_keys_str_mv AT respektanatalia plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT pichkarolina plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT mlyczynskaewa plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT dobrzynkamil plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT ramechristelle plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT kaminskitadeusz plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT smolinskanina plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT dupontjoelle plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells
AT rakagnieszka plasmalevelofomentin1itsexpressionanditsregulationbygonadotropinreleasinghormoneandgonadotropinsinporcineanteriorpituitarycells