Cargando…

Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus

Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads progress to cortical layers to establish func...

Descripción completa

Detalles Bibliográficos
Autores principales: Frank, Manuel, Fechete, Lavinia Ioana, Tedeschi, Francesca, Nadzieja, Marcin, Nørgaard, Malita Malou Malekzadeh, Montiel, Jesus, Andersen, Kasper Røjkjær, Schierup, Mikkel H., Reid, Dugald, Andersen, Stig Uggerhøj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630511/
https://www.ncbi.nlm.nih.gov/pubmed/37935666
http://dx.doi.org/10.1038/s41467-023-42911-1
_version_ 1785132165067964416
author Frank, Manuel
Fechete, Lavinia Ioana
Tedeschi, Francesca
Nadzieja, Marcin
Nørgaard, Malita Malou Malekzadeh
Montiel, Jesus
Andersen, Kasper Røjkjær
Schierup, Mikkel H.
Reid, Dugald
Andersen, Stig Uggerhøj
author_facet Frank, Manuel
Fechete, Lavinia Ioana
Tedeschi, Francesca
Nadzieja, Marcin
Nørgaard, Malita Malou Malekzadeh
Montiel, Jesus
Andersen, Kasper Røjkjær
Schierup, Mikkel H.
Reid, Dugald
Andersen, Stig Uggerhøj
author_sort Frank, Manuel
collection PubMed
description Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads progress to cortical layers to establish functional nodules. Here we use single-cell analysis to define the epidermal and cortical cell populations that respond to and facilitate rhizobium infection. We then identify high-confidence nodulation gene candidates based on their specific expression in these populations, pinpointing genes stably associated with infection across genotypes and time points. We show that one of these, which we name SYMRKL1, encodes a protein with an ectodomain predicted to be nearly identical to that of SYMRK and is required for normal infection thread formation. Our work disentangles cellular processes and transcriptional modules that were previously confounded due to lack of cellular resolution, providing a more detailed understanding of symbiotic interactions.
format Online
Article
Text
id pubmed-10630511
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106305112023-11-07 Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus Frank, Manuel Fechete, Lavinia Ioana Tedeschi, Francesca Nadzieja, Marcin Nørgaard, Malita Malou Malekzadeh Montiel, Jesus Andersen, Kasper Røjkjær Schierup, Mikkel H. Reid, Dugald Andersen, Stig Uggerhøj Nat Commun Article Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads progress to cortical layers to establish functional nodules. Here we use single-cell analysis to define the epidermal and cortical cell populations that respond to and facilitate rhizobium infection. We then identify high-confidence nodulation gene candidates based on their specific expression in these populations, pinpointing genes stably associated with infection across genotypes and time points. We show that one of these, which we name SYMRKL1, encodes a protein with an ectodomain predicted to be nearly identical to that of SYMRK and is required for normal infection thread formation. Our work disentangles cellular processes and transcriptional modules that were previously confounded due to lack of cellular resolution, providing a more detailed understanding of symbiotic interactions. Nature Publishing Group UK 2023-11-07 /pmc/articles/PMC10630511/ /pubmed/37935666 http://dx.doi.org/10.1038/s41467-023-42911-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Frank, Manuel
Fechete, Lavinia Ioana
Tedeschi, Francesca
Nadzieja, Marcin
Nørgaard, Malita Malou Malekzadeh
Montiel, Jesus
Andersen, Kasper Røjkjær
Schierup, Mikkel H.
Reid, Dugald
Andersen, Stig Uggerhøj
Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus
title Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus
title_full Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus
title_fullStr Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus
title_full_unstemmed Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus
title_short Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus
title_sort single-cell analysis identifies genes facilitating rhizobium infection in lotus japonicus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630511/
https://www.ncbi.nlm.nih.gov/pubmed/37935666
http://dx.doi.org/10.1038/s41467-023-42911-1
work_keys_str_mv AT frankmanuel singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT fechetelaviniaioana singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT tedeschifrancesca singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT nadziejamarcin singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT nørgaardmalitamaloumalekzadeh singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT montieljesus singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT andersenkasperrøjkjær singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT schierupmikkelh singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT reiddugald singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus
AT andersenstiguggerhøj singlecellanalysisidentifiesgenesfacilitatingrhizobiuminfectioninlotusjaponicus