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Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens
The forest canopy harbors a diverse array of organisms. However, monitoring their biodiversity poses challenges due to limited accessibility and the vast taxonomic diversity. To address these challenges, we present a novel method for capturing arboreal biodiversity by harnessing stemflow as a source...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630645/ https://www.ncbi.nlm.nih.gov/pubmed/38023308 http://dx.doi.org/10.1016/j.mex.2023.102448 |
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author | Sakata, Ayumi Sado, Tetsuya Oka, Shin-ichiro Ushio, Masayuki Miya, Masaki |
author_facet | Sakata, Ayumi Sado, Tetsuya Oka, Shin-ichiro Ushio, Masayuki Miya, Masaki |
author_sort | Sakata, Ayumi |
collection | PubMed |
description | The forest canopy harbors a diverse array of organisms. However, monitoring their biodiversity poses challenges due to limited accessibility and the vast taxonomic diversity. To address these challenges, we present a novel method for capturing arboreal biodiversity by harnessing stemflow as a source of DNA from organisms inhabiting trees. Our method involves encircling the tree trunk with gauze, directing the stemflow along the gauze into a funnel, and collecting it in a plastic bag. We employed dual collection systems to retrieve environmental DNA (eDNA) from the stemflow: the gauze trap, designed to capture macroscopic biological fragments, and the plastic bag trap, which collected the stemflow itself. The trapped fragments and stemflow were separately filtered, and eDNA was subsequently extracted from the filter membranes. To validate our method, we focused on foliose lichens, which are easily observable on tree surfaces. We performed eDNA metabarcoding and successfully detected a majority of the observed foliose lichen species, including those not identified through visual observation alone. • We have developed a non-invasive and straightforward method for monitoring arboreal biodiversity by collecting eDNA from stemflow, which has been validated using lichens for its efficacy. • This cost-effective approach minimizes disruptions to tree ecosystems and is expected to provide an efficient means of sampling and monitoring arboreal organisms. |
format | Online Article Text |
id | pubmed-10630645 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-106306452023-10-18 Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens Sakata, Ayumi Sado, Tetsuya Oka, Shin-ichiro Ushio, Masayuki Miya, Masaki MethodsX Agricultural and Biological Science The forest canopy harbors a diverse array of organisms. However, monitoring their biodiversity poses challenges due to limited accessibility and the vast taxonomic diversity. To address these challenges, we present a novel method for capturing arboreal biodiversity by harnessing stemflow as a source of DNA from organisms inhabiting trees. Our method involves encircling the tree trunk with gauze, directing the stemflow along the gauze into a funnel, and collecting it in a plastic bag. We employed dual collection systems to retrieve environmental DNA (eDNA) from the stemflow: the gauze trap, designed to capture macroscopic biological fragments, and the plastic bag trap, which collected the stemflow itself. The trapped fragments and stemflow were separately filtered, and eDNA was subsequently extracted from the filter membranes. To validate our method, we focused on foliose lichens, which are easily observable on tree surfaces. We performed eDNA metabarcoding and successfully detected a majority of the observed foliose lichen species, including those not identified through visual observation alone. • We have developed a non-invasive and straightforward method for monitoring arboreal biodiversity by collecting eDNA from stemflow, which has been validated using lichens for its efficacy. • This cost-effective approach minimizes disruptions to tree ecosystems and is expected to provide an efficient means of sampling and monitoring arboreal organisms. Elsevier 2023-10-18 /pmc/articles/PMC10630645/ /pubmed/38023308 http://dx.doi.org/10.1016/j.mex.2023.102448 Text en © 2023 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Agricultural and Biological Science Sakata, Ayumi Sado, Tetsuya Oka, Shin-ichiro Ushio, Masayuki Miya, Masaki Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens |
title | Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens |
title_full | Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens |
title_fullStr | Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens |
title_full_unstemmed | Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens |
title_short | Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens |
title_sort | collection of environmental dna from stemflow for monitoring arboreal biodiversity: preliminary validation using lichens |
topic | Agricultural and Biological Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630645/ https://www.ncbi.nlm.nih.gov/pubmed/38023308 http://dx.doi.org/10.1016/j.mex.2023.102448 |
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