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Protocol for quantifying the germination properties of individual bacterial endospores using PySpore

PySpore is a Python program that tracks the germination of individual bacterial endospores. Here, we present a protocol for segmenting spores and quantifying the germination properties of individual bacterial endospores using PySpore. We describe steps for using GUI-based tools to optimize image pro...

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Detalles Bibliográficos
Autores principales: Ribis, John W., Shen, Aimee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630823/
https://www.ncbi.nlm.nih.gov/pubmed/37910513
http://dx.doi.org/10.1016/j.xpro.2023.102678

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