Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry

Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution...

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Detalles Bibliográficos
Autores principales: Song, Jinyuan, Zhang, Junlei, Ji, Yongtao, Tang, Jianghui, Sheng, Jianpeng, Liang, Tingbo, Bai, Xueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630849/
https://www.ncbi.nlm.nih.gov/pubmed/37910511
http://dx.doi.org/10.1016/j.xpro.2023.102679
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author Song, Jinyuan
Zhang, Junlei
Ji, Yongtao
Tang, Jianghui
Sheng, Jianpeng
Liang, Tingbo
Bai, Xueli
author_facet Song, Jinyuan
Zhang, Junlei
Ji, Yongtao
Tang, Jianghui
Sheng, Jianpeng
Liang, Tingbo
Bai, Xueli
author_sort Song, Jinyuan
collection PubMed
description Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).(1)
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spelling pubmed-106308492023-10-31 Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry Song, Jinyuan Zhang, Junlei Ji, Yongtao Tang, Jianghui Sheng, Jianpeng Liang, Tingbo Bai, Xueli STAR Protoc Protocol Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).(1) Elsevier 2023-10-31 /pmc/articles/PMC10630849/ /pubmed/37910511 http://dx.doi.org/10.1016/j.xpro.2023.102679 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Song, Jinyuan
Zhang, Junlei
Ji, Yongtao
Tang, Jianghui
Sheng, Jianpeng
Liang, Tingbo
Bai, Xueli
Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
title Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
title_full Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
title_fullStr Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
title_full_unstemmed Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
title_short Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
title_sort protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630849/
https://www.ncbi.nlm.nih.gov/pubmed/37910511
http://dx.doi.org/10.1016/j.xpro.2023.102679
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