Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry

Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution...

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Detalles Bibliográficos
Autores principales: Song, Jinyuan, Zhang, Junlei, Ji, Yongtao, Tang, Jianghui, Sheng, Jianpeng, Liang, Tingbo, Bai, Xueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630849/
https://www.ncbi.nlm.nih.gov/pubmed/37910511
http://dx.doi.org/10.1016/j.xpro.2023.102679

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