Cargando…
Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan
Anaplasma marginale (A. marginale), Anaplasma ovis (A. ovis) and Theileria ovis (T. ovis) are among the most commonly reported intracellular tick borne pathogens that infect ruminants across the globe causing huge economic losses. This study aims to report the prevalence and phylogenetic evaluation...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10631641/ https://www.ncbi.nlm.nih.gov/pubmed/37939034 http://dx.doi.org/10.1371/journal.pone.0291302 |
_version_ | 1785132403745882112 |
---|---|
author | Arif, Muhammad Saeed, Suleman Bashir, Ahsan Farooq, Muhammad Nasreen, Nasreen Khan, Adil Asif, Muhammad Khalil, Muhammad Asif Ijaz, Maryam Muqaddas, Hira Mehmood, Naunain Iqbal, Furhan Chen, Chien-Chin |
author_facet | Arif, Muhammad Saeed, Suleman Bashir, Ahsan Farooq, Muhammad Nasreen, Nasreen Khan, Adil Asif, Muhammad Khalil, Muhammad Asif Ijaz, Maryam Muqaddas, Hira Mehmood, Naunain Iqbal, Furhan Chen, Chien-Chin |
author_sort | Arif, Muhammad |
collection | PubMed |
description | Anaplasma marginale (A. marginale), Anaplasma ovis (A. ovis) and Theileria ovis (T. ovis) are among the most commonly reported intracellular tick borne pathogens that infect ruminants across the globe causing huge economic losses. This study aims to report the prevalence and phylogenetic evaluation of these three pathogens infecting sheep and goats (n = 333) that were enrolled from Fort Munro region in Pakistan by using msp1b, msp4 and 18S rRNA genes for A. marginale, A. ovis and T. ovis respectively. Results revealed almost similar infection rates in sheep and goats with an overall prevalence of 11% for A. marginale, 28% for A. ovis and 3% for T. ovis. Concurrent infection was also recorded, however, the number of animals infected with two pathogens (n = 24; 7.2%) was higher than infection with three pathogens (n = 2; 0.6%). Risk factor analysis revealed that sheep reared in small herds had higher A. marginale (P = 0.03) and A. ovis (P = 0.04) infection rates compared to those from large herds. In addition, it was observed that bucks (P ≤ 0.05) and tick-free goats (P ≤ 0.05) exhibited higher A. ovis infection rates than nannies. Phylogenetic analysis of all three pathogens showed that Pakistani isolates were clustered together and were closely related to previously deposited Pakistani isolates as well as with those that were reported from worldwide countries. In conclusion, we are reporting that Pakistani sheep and goats have A. marginale, A. ovis and T. ovis mediated infections and control measures should be taken against them to improve the productivity of the livestock sector. |
format | Online Article Text |
id | pubmed-10631641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-106316412023-11-08 Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan Arif, Muhammad Saeed, Suleman Bashir, Ahsan Farooq, Muhammad Nasreen, Nasreen Khan, Adil Asif, Muhammad Khalil, Muhammad Asif Ijaz, Maryam Muqaddas, Hira Mehmood, Naunain Iqbal, Furhan Chen, Chien-Chin PLoS One Research Article Anaplasma marginale (A. marginale), Anaplasma ovis (A. ovis) and Theileria ovis (T. ovis) are among the most commonly reported intracellular tick borne pathogens that infect ruminants across the globe causing huge economic losses. This study aims to report the prevalence and phylogenetic evaluation of these three pathogens infecting sheep and goats (n = 333) that were enrolled from Fort Munro region in Pakistan by using msp1b, msp4 and 18S rRNA genes for A. marginale, A. ovis and T. ovis respectively. Results revealed almost similar infection rates in sheep and goats with an overall prevalence of 11% for A. marginale, 28% for A. ovis and 3% for T. ovis. Concurrent infection was also recorded, however, the number of animals infected with two pathogens (n = 24; 7.2%) was higher than infection with three pathogens (n = 2; 0.6%). Risk factor analysis revealed that sheep reared in small herds had higher A. marginale (P = 0.03) and A. ovis (P = 0.04) infection rates compared to those from large herds. In addition, it was observed that bucks (P ≤ 0.05) and tick-free goats (P ≤ 0.05) exhibited higher A. ovis infection rates than nannies. Phylogenetic analysis of all three pathogens showed that Pakistani isolates were clustered together and were closely related to previously deposited Pakistani isolates as well as with those that were reported from worldwide countries. In conclusion, we are reporting that Pakistani sheep and goats have A. marginale, A. ovis and T. ovis mediated infections and control measures should be taken against them to improve the productivity of the livestock sector. Public Library of Science 2023-11-08 /pmc/articles/PMC10631641/ /pubmed/37939034 http://dx.doi.org/10.1371/journal.pone.0291302 Text en © 2023 Arif et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Arif, Muhammad Saeed, Suleman Bashir, Ahsan Farooq, Muhammad Nasreen, Nasreen Khan, Adil Asif, Muhammad Khalil, Muhammad Asif Ijaz, Maryam Muqaddas, Hira Mehmood, Naunain Iqbal, Furhan Chen, Chien-Chin Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan |
title | Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan |
title_full | Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan |
title_fullStr | Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan |
title_full_unstemmed | Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan |
title_short | Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan |
title_sort | molecular prevalence and phylogeny of anaplasma marginale, anaplasma ovis and theileria ovis in goats and sheep enrolled from a hill station in punjab, pakistan |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10631641/ https://www.ncbi.nlm.nih.gov/pubmed/37939034 http://dx.doi.org/10.1371/journal.pone.0291302 |
work_keys_str_mv | AT arifmuhammad molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT saeedsuleman molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT bashirahsan molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT farooqmuhammad molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT nasreennasreen molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT khanadil molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT asifmuhammad molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT khalilmuhammadasif molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT ijazmaryam molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT muqaddashira molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT mehmoodnaunain molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT iqbalfurhan molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan AT chenchienchin molecularprevalenceandphylogenyofanaplasmamarginaleanaplasmaovisandtheileriaovisingoatsandsheepenrolledfromahillstationinpunjabpakistan |