Cargando…
Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance
Vancomycin, is widely used against methicillin-resistant bacterial infections. However, Vancomycin accumulation causes nephrotoxicity which leads to an impairment in the filtration mechanisms of kidney. Traditional herbal medicines hold potential for treatment of drug-induced nephrotoxicity. Herein,...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10631690/ https://www.ncbi.nlm.nih.gov/pubmed/37939153 http://dx.doi.org/10.1371/journal.pone.0293605 |
_version_ | 1785132415594790912 |
---|---|
author | Balkrishna, Acharya Sharma, Sonam Gohel, Vivek Kumari, Ankita Rawat, Malini Maity, Madhulina Sinha, Sandeep Dev, Rishabh Varshney, Anurag |
author_facet | Balkrishna, Acharya Sharma, Sonam Gohel, Vivek Kumari, Ankita Rawat, Malini Maity, Madhulina Sinha, Sandeep Dev, Rishabh Varshney, Anurag |
author_sort | Balkrishna, Acharya |
collection | PubMed |
description | Vancomycin, is widely used against methicillin-resistant bacterial infections. However, Vancomycin accumulation causes nephrotoxicity which leads to an impairment in the filtration mechanisms of kidney. Traditional herbal medicines hold potential for treatment of drug-induced nephrotoxicity. Herein, we investigated protective properties of plant-based medicine Renogrit against Vancomycin-induced kidney injury. Phytometabolite analysis of Renogrit was performed by UHPLC. Spheroids formed from human proximal tubular cell (HK-2) were used for in vitro evaluation of Vancomycin-induced alterations in cell viability, P-gp functionality, NAG, KIM-1 levels, and mRNA expression of NGAL and MMP-7. The in vivo efficacy of Renogrit against Vancomycin-induced nephrotoxicity was further evaluated in Sprague-Dawley (SD) rats by measurement of BUN, serum creatinine, and their respective clearances. Moreover, eGFR, kidney-to-body weight ratio, GSH/GSSG ratio, KIM-1, NAG levels and mRNA expression of KIM-1 and osteopontin were also analyzed. Changes in histopathology of kidney and hematological parameters were also observed. Renogrit treatment led to an increase in cell viability, normalization of P-gp functionality, decrease in levels of NAG, KIM-1, and reduction in mRNA expression of NGAL and MMP-7. In Vancomycin-challenged SD rats, Renogrit treatment normalized altered kidney functions, histological, and hematological parameters. Our findings revealed that Renogrit holds a clinico-therapeutic potential for alleviating Vancomycin-associated nephrotoxicity. |
format | Online Article Text |
id | pubmed-10631690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-106316902023-11-08 Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance Balkrishna, Acharya Sharma, Sonam Gohel, Vivek Kumari, Ankita Rawat, Malini Maity, Madhulina Sinha, Sandeep Dev, Rishabh Varshney, Anurag PLoS One Research Article Vancomycin, is widely used against methicillin-resistant bacterial infections. However, Vancomycin accumulation causes nephrotoxicity which leads to an impairment in the filtration mechanisms of kidney. Traditional herbal medicines hold potential for treatment of drug-induced nephrotoxicity. Herein, we investigated protective properties of plant-based medicine Renogrit against Vancomycin-induced kidney injury. Phytometabolite analysis of Renogrit was performed by UHPLC. Spheroids formed from human proximal tubular cell (HK-2) were used for in vitro evaluation of Vancomycin-induced alterations in cell viability, P-gp functionality, NAG, KIM-1 levels, and mRNA expression of NGAL and MMP-7. The in vivo efficacy of Renogrit against Vancomycin-induced nephrotoxicity was further evaluated in Sprague-Dawley (SD) rats by measurement of BUN, serum creatinine, and their respective clearances. Moreover, eGFR, kidney-to-body weight ratio, GSH/GSSG ratio, KIM-1, NAG levels and mRNA expression of KIM-1 and osteopontin were also analyzed. Changes in histopathology of kidney and hematological parameters were also observed. Renogrit treatment led to an increase in cell viability, normalization of P-gp functionality, decrease in levels of NAG, KIM-1, and reduction in mRNA expression of NGAL and MMP-7. In Vancomycin-challenged SD rats, Renogrit treatment normalized altered kidney functions, histological, and hematological parameters. Our findings revealed that Renogrit holds a clinico-therapeutic potential for alleviating Vancomycin-associated nephrotoxicity. Public Library of Science 2023-11-08 /pmc/articles/PMC10631690/ /pubmed/37939153 http://dx.doi.org/10.1371/journal.pone.0293605 Text en © 2023 Balkrishna et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Balkrishna, Acharya Sharma, Sonam Gohel, Vivek Kumari, Ankita Rawat, Malini Maity, Madhulina Sinha, Sandeep Dev, Rishabh Varshney, Anurag Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance |
title | Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance |
title_full | Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance |
title_fullStr | Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance |
title_full_unstemmed | Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance |
title_short | Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance |
title_sort | renogrit attenuates vancomycin-induced nephrotoxicity in human renal spheroids and in sprague-dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10631690/ https://www.ncbi.nlm.nih.gov/pubmed/37939153 http://dx.doi.org/10.1371/journal.pone.0293605 |
work_keys_str_mv | AT balkrishnaacharya renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT sharmasonam renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT gohelvivek renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT kumariankita renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT rawatmalini renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT maitymadhulina renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT sinhasandeep renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT devrishabh renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance AT varshneyanurag renogritattenuatesvancomycininducednephrotoxicityinhumanrenalspheroidsandinspraguedawleyratsbyregulatingkidneyinjurybiomarkersandcreatinineureaclearance |