Cargando…

Way to efficient microbial paclitaxel mass production

The microbial synthesis of paclitaxel is attractive for its short-cycle, cost-effectiveness, and sustainability. However, low paclitaxel productivity, depleted capacity during subculture and storage, and unclear biosynthesis mechanisms restrain industrial microbial synthesis. Along with the isolatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Chenyue, Qi, Yanli, Sun, Zhongke, Jiang, Mengwan, Li, Chengwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632112/
https://www.ncbi.nlm.nih.gov/pubmed/37954482
http://dx.doi.org/10.1016/j.synbio.2023.10.002
_version_ 1785132508008939520
author Li, Chenyue
Qi, Yanli
Sun, Zhongke
Jiang, Mengwan
Li, Chengwei
author_facet Li, Chenyue
Qi, Yanli
Sun, Zhongke
Jiang, Mengwan
Li, Chengwei
author_sort Li, Chenyue
collection PubMed
description The microbial synthesis of paclitaxel is attractive for its short-cycle, cost-effectiveness, and sustainability. However, low paclitaxel productivity, depleted capacity during subculture and storage, and unclear biosynthesis mechanisms restrain industrial microbial synthesis. Along with the isolation of various paclitaxel-producing microorganisms and the development of versatile molecular tools, tremendous promises for microbial paclitaxel synthesis have become increasingly prominent. In this review, we summarize the progress of microbial synthesis of paclitaxel in recent years, focusing on paclitaxel-producing endophytes and representative engineering microorganism hosts that were used as chassis for paclitaxel precursor synthesis. Numerous wide-type microbes can manufacture paclitaxel, and fermentation process optimization and strain improvement can greatly enhance the productivity. Engineered microbes can efficiently synthesize precursors of paclitaxel by introducing exogenous synthetic pathway. Mining paclitaxel synthetic pathways and genetic manipulation of endophytes will accelerate the construction of microbial cell factories, indefinitely contributing to paclitaxel mass production by microbes. This review emphasizes the potential and provides solutions for efficient microbial paclitaxel mass production.
format Online
Article
Text
id pubmed-10632112
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher KeAi Publishing
record_format MEDLINE/PubMed
spelling pubmed-106321122023-11-10 Way to efficient microbial paclitaxel mass production Li, Chenyue Qi, Yanli Sun, Zhongke Jiang, Mengwan Li, Chengwei Synth Syst Biotechnol Review Article The microbial synthesis of paclitaxel is attractive for its short-cycle, cost-effectiveness, and sustainability. However, low paclitaxel productivity, depleted capacity during subculture and storage, and unclear biosynthesis mechanisms restrain industrial microbial synthesis. Along with the isolation of various paclitaxel-producing microorganisms and the development of versatile molecular tools, tremendous promises for microbial paclitaxel synthesis have become increasingly prominent. In this review, we summarize the progress of microbial synthesis of paclitaxel in recent years, focusing on paclitaxel-producing endophytes and representative engineering microorganism hosts that were used as chassis for paclitaxel precursor synthesis. Numerous wide-type microbes can manufacture paclitaxel, and fermentation process optimization and strain improvement can greatly enhance the productivity. Engineered microbes can efficiently synthesize precursors of paclitaxel by introducing exogenous synthetic pathway. Mining paclitaxel synthetic pathways and genetic manipulation of endophytes will accelerate the construction of microbial cell factories, indefinitely contributing to paclitaxel mass production by microbes. This review emphasizes the potential and provides solutions for efficient microbial paclitaxel mass production. KeAi Publishing 2023-10-19 /pmc/articles/PMC10632112/ /pubmed/37954482 http://dx.doi.org/10.1016/j.synbio.2023.10.002 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review Article
Li, Chenyue
Qi, Yanli
Sun, Zhongke
Jiang, Mengwan
Li, Chengwei
Way to efficient microbial paclitaxel mass production
title Way to efficient microbial paclitaxel mass production
title_full Way to efficient microbial paclitaxel mass production
title_fullStr Way to efficient microbial paclitaxel mass production
title_full_unstemmed Way to efficient microbial paclitaxel mass production
title_short Way to efficient microbial paclitaxel mass production
title_sort way to efficient microbial paclitaxel mass production
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632112/
https://www.ncbi.nlm.nih.gov/pubmed/37954482
http://dx.doi.org/10.1016/j.synbio.2023.10.002
work_keys_str_mv AT lichenyue waytoefficientmicrobialpaclitaxelmassproduction
AT qiyanli waytoefficientmicrobialpaclitaxelmassproduction
AT sunzhongke waytoefficientmicrobialpaclitaxelmassproduction
AT jiangmengwan waytoefficientmicrobialpaclitaxelmassproduction
AT lichengwei waytoefficientmicrobialpaclitaxelmassproduction