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Preparation of Whole-mount Mouse Islets on Vascular Extracellular Matrix for Live Islet Cell Microscopy

Pancreatic islet β cells preferentially secrete insulin toward the plasma membrane, making contact with the capillary extracellular matrix (ECM). Isolated islets separated from the exocrine acinar cells are the best system for cell biology studies of primary β cells, whereas isolated islets lose the...

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Detalles Bibliográficos
Autores principales: Ho, Kung-Hsien, Gu, Guoqiang, Kaverina, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632159/
https://www.ncbi.nlm.nih.gov/pubmed/37969764
http://dx.doi.org/10.21769/BioProtoc.4868