Cargando…

Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass—Tips and Considerations

The precise and rapid detection of fungi is important in various fields, including clinics, industry, and agriculture. While sequencing universal DNA barcodes remains the standard method for species identification and phylogenetic analysis, a significant bottleneck has been the labor-intensive and t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jeon, Hosung, Son, Hokyoung, Min, Kyunghun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bio-Protocol 2023
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632164/ https://www.ncbi.nlm.nih.gov/pubmed/37969756 http://dx.doi.org/10.21769/BioProtoc.4889

Ejemplares similares

Application of direct PCR for phylogenetic analysis of Fusarium fujikuroi species complex isolated from rice seeds
por: Jeon, Hosung, et al.
Publicado: (2023)

PCR-tips for rapid diagnosis of bacterial pathogens
por: Gimenez, Claudia, et al.
Publicado: (2022)

Micro-scale Experimental System Coupled with Fluorescence-based Estimation of Fungal Biomass to Study Utilisation of Plant Substrates
por: Németh, Julianna B., et al.
Publicado: (2021)

Infectivity and stress tolerance traits affect community assembly of plant pathogenic fungi
por: Choi, Soyoung, et al.
Publicado: (2023)

Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy
por: Dzurendová, Simona, et al.
Publicado: (2021)

An optimized protocol for stepwise optimization of real-time RT-PCR analysis
por: Zhao, Fangzhou, et al.
Publicado: (2021)

Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways
por: Bookout, Angie L., et al.
Publicado: (2003)

Production of L-carnitine-enriched edible filamentous fungal biomass through submerged cultivation
por: Rousta, Neda, et al.
Publicado: (2021)

Assessing the detailed time course of perceptual sensitivity change in perceptual learning
por: Zhang, Pan, et al.
Publicado: (2019)

Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol
por: Chovancova, Petra, et al.
Publicado: (2017)

Direct observation of the actin filament by tip-scan atomic force microscopy
por: Narita, Akihiro, et al.
Publicado: (2016)

Transcranial direct current stimulation with functional magnetic resonance imaging: a detailed validation and operational guide
por: Nardo, Davide, et al.
Publicado: (2023)

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates
por: Sanchis, Joaquin, et al.
Publicado: (2008)

Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex
por: Vera-Salmoral, Eduardo, et al.
Publicado: (2023)

Determining the linear correlation between dielectric spectroscopy and viable biomass concentration in filamentous fungal fermentations
por: Magnússon, Atli, et al.
Publicado: (2023)

Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
por: Kai, Shinichi, et al.
Publicado: (2019)

A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA
por: Jung, Ulrike, et al.
Publicado: (2013)

Identification and Characterization of Fungal Pathogens Associated with Boxwood Diseases in the Republic of Korea
por: Shin, Soobin, et al.
Publicado: (2022)

A method for filamentous fungal growth and sample preparation aimed at more consistent MALDI-TOF MS spectra despite variations in growth rates and/or incubation times
por: Reeve, Michael A, et al.
Publicado: (2019)

Fungal oxylipins direct programmed developmental switches in filamentous fungi
por: Niu, Mengyao, et al.
Publicado: (2020)

Direct Visualization of Fungal Burden in Filamentous Fungus-Infected Silkworms
por: Yu, Yidong, et al.
Publicado: (2021)

Measuring the force of the tip of the elephants trunk
por: Cornette, Raphaël, et al.
Publicado: (2022)

A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
por: Wu, Wu, et al.
Publicado: (2005)

An improved RT-qPCR method for direct quantification of enveloped RNA viruses
por: Gregorova, Pavlina, et al.
Publicado: (2022)

Species-resolved sequencing of low-biomass or degraded microbiomes using 2bRAD-M
por: Sun, Zheng, et al.
Publicado: (2022)

De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data
por: DiGuistini, Scott, et al.
Publicado: (2009)

Thick filament‐associated myosin undergoes frequent replacement at the tip of the thick filament
por: Ichimura, Emi, et al.
Publicado: (2022)

Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum
por: Lee, Nahyun, et al.
Publicado: (2022)

Practical data handling pipeline improves performance of qPCR-based circulating miRNA measurements
por: de Ronde, Maurice W.J., et al.
Publicado: (2017)

Constructing a Plasma Nutriproteome for Population Assessment: Analytical Considerations
por: Kim, Hyunju, et al.
Publicado: (2022)

Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance
por: Lebedev, Alexandre V., et al.
Publicado: (2008)

Direct elicitation of template concentration from quantification cycle (C(q)) distributions in digital PCR
por: Mojtahedi, Mitra, et al.
Publicado: (2014)

A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
por: Song, Fangchao, et al.
Publicado: (2021)

Fungal Cytochrome P450s and the P450 Complement (CYPome) of Fusarium graminearum
por: Shin, Jiyoung, et al.
Publicado: (2018)

Method for ultrastructural fine details of plant cuticles by transmission electron microscopy
por: Guignard, Gaëtan
Publicado: (2021)

A rapid and simple quantitative method for specific detection of smaller coterminal RNA by PCR (DeSCo-PCR): application to the detection of viral subgenomic RNAs
por: Kanodia, Pulkit, et al.
Publicado: (2020)

Direct PCR Offers a Fast and Reliable Alternative to Conventional DNA Isolation Methods for Gut Microbiomes
por: Videvall, Elin, et al.
Publicado: (2017)

Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
por: Aird, Daniel, et al.
Publicado: (2011)

Choreography for nucleosomes: the conformational freedom of the nucleosomal filament and its limitations
por: Engelhardt, Mogens
Publicado: (2007)

Estimating Sizes of Key Populations at the National Level: Considerations for Study Design and Analysis
por: Edwards, Jessie K., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_vtvu90tr5o2i7gng158eb3cbor