Cargando…

Three dimensional (3D) gingival models in periodontal research: a systematic review

The aim of this study is to systematically appraise the evidence on available full thickness 3D gingival and mucosal models (3D culture in scaffold base system) and their application in periodontal and peri-implant research. This study involved a systematic review of twenty-two studies obtained from...

Descripción completa

Detalles Bibliográficos
Autores principales: AlFatlawi, Z., Huang, M., Chau, D.Y.S., D’Aiuto, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632299/
https://www.ncbi.nlm.nih.gov/pubmed/37938480
http://dx.doi.org/10.1007/s10856-023-06761-z
_version_ 1785146135198826496
author AlFatlawi, Z.
Huang, M.
Chau, D.Y.S.
D’Aiuto, F.
author_facet AlFatlawi, Z.
Huang, M.
Chau, D.Y.S.
D’Aiuto, F.
author_sort AlFatlawi, Z.
collection PubMed
description The aim of this study is to systematically appraise the evidence on available full thickness 3D gingival and mucosal models (3D culture in scaffold base system) and their application in periodontal and peri-implant research. This study involved a systematic review of twenty-two studies obtained from searching from five electronic databases: MEDLINE-OVID, EMBASE, EBSCOhost, Web of Science Core Collection and LILACS, as well as a hand search of eligible articles up to September 2022. A total of 2338 studies were initially identified, after removal of duplicates (573), abstracts/title selection (1765), and full text screening (95), twenty-two studies were included, thirty-seven models were identified. Several cellular markers were reported by the studies included. The expression of keratinocytes differentiation markers (K4, K5, K10, K13, K14, K16, K17, K18, K19, involucrin, laminin5), proliferation marker (Ki67, CD90), and vimentin, Type I, II and IV collagen produced by fibroblasts were investigated in thirty models. No quantitative analyses were performed, and results of the review confirmed a substantial level of heterogeneity across experiments. In conclusion, there is currently insufficient evidence to conclude that the available 3D gingival and mucosal models can entirely recapitulate the human gingival tissue/mucosa and provide a useful research tool for periodontal and peri-implant research. This review also highlighted the lack of a standardized protocol to construct and characterize 3D gingival models. A new protocol is proposed for the characterization of in vitro gingival models for future research.
format Online
Article
Text
id pubmed-10632299
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-106322992023-11-14 Three dimensional (3D) gingival models in periodontal research: a systematic review AlFatlawi, Z. Huang, M. Chau, D.Y.S. D’Aiuto, F. J Mater Sci Mater Med Tissue Engineering Constructs and Cell Substrates The aim of this study is to systematically appraise the evidence on available full thickness 3D gingival and mucosal models (3D culture in scaffold base system) and their application in periodontal and peri-implant research. This study involved a systematic review of twenty-two studies obtained from searching from five electronic databases: MEDLINE-OVID, EMBASE, EBSCOhost, Web of Science Core Collection and LILACS, as well as a hand search of eligible articles up to September 2022. A total of 2338 studies were initially identified, after removal of duplicates (573), abstracts/title selection (1765), and full text screening (95), twenty-two studies were included, thirty-seven models were identified. Several cellular markers were reported by the studies included. The expression of keratinocytes differentiation markers (K4, K5, K10, K13, K14, K16, K17, K18, K19, involucrin, laminin5), proliferation marker (Ki67, CD90), and vimentin, Type I, II and IV collagen produced by fibroblasts were investigated in thirty models. No quantitative analyses were performed, and results of the review confirmed a substantial level of heterogeneity across experiments. In conclusion, there is currently insufficient evidence to conclude that the available 3D gingival and mucosal models can entirely recapitulate the human gingival tissue/mucosa and provide a useful research tool for periodontal and peri-implant research. This review also highlighted the lack of a standardized protocol to construct and characterize 3D gingival models. A new protocol is proposed for the characterization of in vitro gingival models for future research. Springer US 2023-11-08 2023 /pmc/articles/PMC10632299/ /pubmed/37938480 http://dx.doi.org/10.1007/s10856-023-06761-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Tissue Engineering Constructs and Cell Substrates
AlFatlawi, Z.
Huang, M.
Chau, D.Y.S.
D’Aiuto, F.
Three dimensional (3D) gingival models in periodontal research: a systematic review
title Three dimensional (3D) gingival models in periodontal research: a systematic review
title_full Three dimensional (3D) gingival models in periodontal research: a systematic review
title_fullStr Three dimensional (3D) gingival models in periodontal research: a systematic review
title_full_unstemmed Three dimensional (3D) gingival models in periodontal research: a systematic review
title_short Three dimensional (3D) gingival models in periodontal research: a systematic review
title_sort three dimensional (3d) gingival models in periodontal research: a systematic review
topic Tissue Engineering Constructs and Cell Substrates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632299/
https://www.ncbi.nlm.nih.gov/pubmed/37938480
http://dx.doi.org/10.1007/s10856-023-06761-z
work_keys_str_mv AT alfatlawiz threedimensional3dgingivalmodelsinperiodontalresearchasystematicreview
AT huangm threedimensional3dgingivalmodelsinperiodontalresearchasystematicreview
AT chaudys threedimensional3dgingivalmodelsinperiodontalresearchasystematicreview
AT daiutof threedimensional3dgingivalmodelsinperiodontalresearchasystematicreview