Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process

Naïve pluripotent state can be obtained by several strategies from various types of cells, in which the cell fate roadmap as well as key biological events involved in the journey have been described in detail. Here, we carefully explored the chromatin accessibility dynamics during the primed-to-naïv...

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Autores principales: Tu, Zhifen, Bi, Yan, Mao, Tengyan, Wang, Hong, Gao, Shaorong, Wang, Yixuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632355/
https://www.ncbi.nlm.nih.gov/pubmed/37938437
http://dx.doi.org/10.1186/s13619-023-00179-2
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author Tu, Zhifen
Bi, Yan
Mao, Tengyan
Wang, Hong
Gao, Shaorong
Wang, Yixuan
author_facet Tu, Zhifen
Bi, Yan
Mao, Tengyan
Wang, Hong
Gao, Shaorong
Wang, Yixuan
author_sort Tu, Zhifen
collection PubMed
description Naïve pluripotent state can be obtained by several strategies from various types of cells, in which the cell fate roadmap as well as key biological events involved in the journey have been described in detail. Here, we carefully explored the chromatin accessibility dynamics during the primed-to-naïve transition by adopting a dual fluorescent reporter system and the assay for transposase-accessible chromatin (ATAC)-seq. Our results revealed critical chromatin remodeling events and highlight the discordance between chromatin accessibility and transcriptional activity. We further demonstrate that the differential epigenetic modifications and transcription factor (TF) activities may play a critical role in regulating gene expression, and account for the observed variations in gene expression despite similar chromatin landscapes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-023-00179-2.
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spelling pubmed-106323552023-11-10 Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process Tu, Zhifen Bi, Yan Mao, Tengyan Wang, Hong Gao, Shaorong Wang, Yixuan Cell Regen Research Article Naïve pluripotent state can be obtained by several strategies from various types of cells, in which the cell fate roadmap as well as key biological events involved in the journey have been described in detail. Here, we carefully explored the chromatin accessibility dynamics during the primed-to-naïve transition by adopting a dual fluorescent reporter system and the assay for transposase-accessible chromatin (ATAC)-seq. Our results revealed critical chromatin remodeling events and highlight the discordance between chromatin accessibility and transcriptional activity. We further demonstrate that the differential epigenetic modifications and transcription factor (TF) activities may play a critical role in regulating gene expression, and account for the observed variations in gene expression despite similar chromatin landscapes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-023-00179-2. Springer Nature Singapore 2023-11-08 /pmc/articles/PMC10632355/ /pubmed/37938437 http://dx.doi.org/10.1186/s13619-023-00179-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Tu, Zhifen
Bi, Yan
Mao, Tengyan
Wang, Hong
Gao, Shaorong
Wang, Yixuan
Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process
title Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process
title_full Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process
title_fullStr Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process
title_full_unstemmed Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process
title_short Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process
title_sort discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632355/
https://www.ncbi.nlm.nih.gov/pubmed/37938437
http://dx.doi.org/10.1186/s13619-023-00179-2
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