Cargando…
The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality
The major limitation of the widespread use of IVP derived embryos is their consistent deficiencies in vitality when compared with their ex vivo derived counterparts. Although embryo metabolism is considered a useful metric of embryo quality, research connecting mitochondrial function with the develo...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632430/ https://www.ncbi.nlm.nih.gov/pubmed/37938581 http://dx.doi.org/10.1038/s41598-023-45691-2 |
_version_ | 1785132574966808576 |
---|---|
author | Kurzella, Jessica Miskel, Dennis Rings, Franca Tholen, Ernst Tesfaye, Dawit Schellander, Karl Salilew-Wondim, Dessie Held-Hoelker, Eva Große-Brinkhaus, Christine Hoelker, Michael |
author_facet | Kurzella, Jessica Miskel, Dennis Rings, Franca Tholen, Ernst Tesfaye, Dawit Schellander, Karl Salilew-Wondim, Dessie Held-Hoelker, Eva Große-Brinkhaus, Christine Hoelker, Michael |
author_sort | Kurzella, Jessica |
collection | PubMed |
description | The major limitation of the widespread use of IVP derived embryos is their consistent deficiencies in vitality when compared with their ex vivo derived counterparts. Although embryo metabolism is considered a useful metric of embryo quality, research connecting mitochondrial function with the developmental capacity of embryos is still lacking. Therefore, the aim of the present study was to analyse bovine embryo respiration signatures in relation to developmental capacity. This was achieved by taking advantage of two generally accepted metrics for developmental capacity: (I) environmental conditions during development (vivo vs. vitro) and (II) developmental kinetics (day 7 vs. day 8 blastocysts). Our study showed that the developmental environment affected total embryo oxygen consumption while different morphokinetics illustrating the embryo qualities correlate with maximal mitochondrial respiration, mitochondrial spare capacity, ATP-linked respiration as well as efficiency of ATP generation. This respiration fingerprint for high embryo quality is reflected by relatively lower lipid contents and relatively higher ROS contents. In summary, the results of the present study extend the existing knowledge on the relationship between bovine embryo quality and the signature of mitochondrial respiration by considering contrasting developmental environments as well as different embryo morphokinetics. |
format | Online Article Text |
id | pubmed-10632430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106324302023-11-10 The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality Kurzella, Jessica Miskel, Dennis Rings, Franca Tholen, Ernst Tesfaye, Dawit Schellander, Karl Salilew-Wondim, Dessie Held-Hoelker, Eva Große-Brinkhaus, Christine Hoelker, Michael Sci Rep Article The major limitation of the widespread use of IVP derived embryos is their consistent deficiencies in vitality when compared with their ex vivo derived counterparts. Although embryo metabolism is considered a useful metric of embryo quality, research connecting mitochondrial function with the developmental capacity of embryos is still lacking. Therefore, the aim of the present study was to analyse bovine embryo respiration signatures in relation to developmental capacity. This was achieved by taking advantage of two generally accepted metrics for developmental capacity: (I) environmental conditions during development (vivo vs. vitro) and (II) developmental kinetics (day 7 vs. day 8 blastocysts). Our study showed that the developmental environment affected total embryo oxygen consumption while different morphokinetics illustrating the embryo qualities correlate with maximal mitochondrial respiration, mitochondrial spare capacity, ATP-linked respiration as well as efficiency of ATP generation. This respiration fingerprint for high embryo quality is reflected by relatively lower lipid contents and relatively higher ROS contents. In summary, the results of the present study extend the existing knowledge on the relationship between bovine embryo quality and the signature of mitochondrial respiration by considering contrasting developmental environments as well as different embryo morphokinetics. Nature Publishing Group UK 2023-11-08 /pmc/articles/PMC10632430/ /pubmed/37938581 http://dx.doi.org/10.1038/s41598-023-45691-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kurzella, Jessica Miskel, Dennis Rings, Franca Tholen, Ernst Tesfaye, Dawit Schellander, Karl Salilew-Wondim, Dessie Held-Hoelker, Eva Große-Brinkhaus, Christine Hoelker, Michael The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality |
title | The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality |
title_full | The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality |
title_fullStr | The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality |
title_full_unstemmed | The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality |
title_short | The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality |
title_sort | mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632430/ https://www.ncbi.nlm.nih.gov/pubmed/37938581 http://dx.doi.org/10.1038/s41598-023-45691-2 |
work_keys_str_mv | AT kurzellajessica themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT miskeldennis themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT ringsfranca themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT tholenernst themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT tesfayedawit themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT schellanderkarl themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT salilewwondimdessie themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT heldhoelkereva themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT großebrinkhauschristine themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT hoelkermichael themitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT kurzellajessica mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT miskeldennis mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT ringsfranca mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT tholenernst mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT tesfayedawit mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT schellanderkarl mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT salilewwondimdessie mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT heldhoelkereva mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT großebrinkhauschristine mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality AT hoelkermichael mitochondrialrespirationsignatureofthebovineblastocystreflectsbothenvironmentalconditionsofdevelopmentaswellasembryoquality |