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Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus.

Gold nanoparticles (AuNPs) have emerged as a promising application in aquaculture. Their nano-sized dimensions, comparable to pathogens offer potential solutions for combating antibiotic resistance. In this study, AuNPs were synthesized by using polychaetes, Marphysa moribidii as the bio-reducing ag...

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Autores principales: Abu Hassan, Mohamad Sofi, Elias, Nurul Ashikin, Hassan, Marina, Rahmah, Sharifah, Wan Ismail, Wan Iryani, Harun, Noor Aniza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632522/
https://www.ncbi.nlm.nih.gov/pubmed/37954386
http://dx.doi.org/10.1016/j.heliyon.2023.e21663
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author Abu Hassan, Mohamad Sofi
Elias, Nurul Ashikin
Hassan, Marina
Rahmah, Sharifah
Wan Ismail, Wan Iryani
Harun, Noor Aniza
author_facet Abu Hassan, Mohamad Sofi
Elias, Nurul Ashikin
Hassan, Marina
Rahmah, Sharifah
Wan Ismail, Wan Iryani
Harun, Noor Aniza
author_sort Abu Hassan, Mohamad Sofi
collection PubMed
description Gold nanoparticles (AuNPs) have emerged as a promising application in aquaculture. Their nano-sized dimensions, comparable to pathogens offer potential solutions for combating antibiotic resistance. In this study, AuNPs were synthesized by using polychaetes, Marphysa moribidii as the bio-reducing agent. Modifications were made to reduce agglomeration in green-synthesized AuNPs through ultrasonication. The antibacterial activities of AuNPs against V. parahaemolyticus were evaluated. The physicochemical characteristics of the green synthesized AuNPs were comprehensively investigated. The successful formation of AuNPs was confirmed by the appearance of a red ruby colour and the presence of surface Plasmon resonance (SPR) absorption peaks at 530 nm as observed from UV–vis spectroscopy. Scanning electron microscopy (SEM) revealed spherical-shaped AuNPs with some agglomerations. Transmission electron microscopy (TEM) showed particle size of AuNPs ranging from 10 nm to 60 nm, meanwhile dynamic light scattering (DLS) analysis indicated an average particle size of 24.36 nm. X-ray diffraction (XRD) analysis confirmed the high crystallinity of AuNPs, and no AuNPs were detected in the polychaetes extracts prior to synthesis. A brief ultrasonication significantly reduced the tendencies for AuNPs to coalesce. The green-synthesized AuNPs demonstrated a remarkable antibacterial efficacy against V. parahaemolyticus. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests revealed that a concentration of 0.3 g/ml of AuNPs effectively inhibited V. parahaemolyticus. These findings highlighted the potential of green-synthesized AuNPs as antibacterial agents for the prevention and management of AHPND in aquaculture.
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spelling pubmed-106325222023-11-10 Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus. Abu Hassan, Mohamad Sofi Elias, Nurul Ashikin Hassan, Marina Rahmah, Sharifah Wan Ismail, Wan Iryani Harun, Noor Aniza Heliyon Research Article Gold nanoparticles (AuNPs) have emerged as a promising application in aquaculture. Their nano-sized dimensions, comparable to pathogens offer potential solutions for combating antibiotic resistance. In this study, AuNPs were synthesized by using polychaetes, Marphysa moribidii as the bio-reducing agent. Modifications were made to reduce agglomeration in green-synthesized AuNPs through ultrasonication. The antibacterial activities of AuNPs against V. parahaemolyticus were evaluated. The physicochemical characteristics of the green synthesized AuNPs were comprehensively investigated. The successful formation of AuNPs was confirmed by the appearance of a red ruby colour and the presence of surface Plasmon resonance (SPR) absorption peaks at 530 nm as observed from UV–vis spectroscopy. Scanning electron microscopy (SEM) revealed spherical-shaped AuNPs with some agglomerations. Transmission electron microscopy (TEM) showed particle size of AuNPs ranging from 10 nm to 60 nm, meanwhile dynamic light scattering (DLS) analysis indicated an average particle size of 24.36 nm. X-ray diffraction (XRD) analysis confirmed the high crystallinity of AuNPs, and no AuNPs were detected in the polychaetes extracts prior to synthesis. A brief ultrasonication significantly reduced the tendencies for AuNPs to coalesce. The green-synthesized AuNPs demonstrated a remarkable antibacterial efficacy against V. parahaemolyticus. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests revealed that a concentration of 0.3 g/ml of AuNPs effectively inhibited V. parahaemolyticus. These findings highlighted the potential of green-synthesized AuNPs as antibacterial agents for the prevention and management of AHPND in aquaculture. Elsevier 2023-10-28 /pmc/articles/PMC10632522/ /pubmed/37954386 http://dx.doi.org/10.1016/j.heliyon.2023.e21663 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Abu Hassan, Mohamad Sofi
Elias, Nurul Ashikin
Hassan, Marina
Rahmah, Sharifah
Wan Ismail, Wan Iryani
Harun, Noor Aniza
Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus.
title Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus.
title_full Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus.
title_fullStr Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus.
title_full_unstemmed Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus.
title_short Polychaeta-mediated synthesis of gold nanoparticles: A potential antibacterial agent against Acute Hepatopancreatic Necrosis Disease (AHPND)–causing bacteria, Vibrio parahaemolyticus.
title_sort polychaeta-mediated synthesis of gold nanoparticles: a potential antibacterial agent against acute hepatopancreatic necrosis disease (ahpnd)–causing bacteria, vibrio parahaemolyticus.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632522/
https://www.ncbi.nlm.nih.gov/pubmed/37954386
http://dx.doi.org/10.1016/j.heliyon.2023.e21663
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