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Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating
In recent years, additive manufacturing techniques have been used to fabricate 3D titanium (Ti)-based scaffolds for production of desirable complex shapes. However, insufficient osteointegration of porous Ti-based scaffolds can elicit long-term complications (e.g., aseptic loosening) and need furthe...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632765/ https://www.ncbi.nlm.nih.gov/pubmed/37954425 http://dx.doi.org/10.1039/d3ra05814j |
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author | Zhang, Bo Feng, Jun Chen, Shuo Liao, Ruohan Zhang, Chengdong Luo, Xuwei Yang, Zelong Xiao, Dongqin He, Kui Duan, Ke |
author_facet | Zhang, Bo Feng, Jun Chen, Shuo Liao, Ruohan Zhang, Chengdong Luo, Xuwei Yang, Zelong Xiao, Dongqin He, Kui Duan, Ke |
author_sort | Zhang, Bo |
collection | PubMed |
description | In recent years, additive manufacturing techniques have been used to fabricate 3D titanium (Ti)-based scaffolds for production of desirable complex shapes. However, insufficient osteointegration of porous Ti-based scaffolds can elicit long-term complications (e.g., aseptic loosening) and need further revision surgery. In this study, a magnesium (Mg)-incorporating tantalum (Ta) coating was deposited on a 3D Ti6Al4V scaffold using a sol–gel method for enhancing its osteogenic properties. To evaluate the biofunction of this surface, bone mesenchymal stem cells and rabbit femoral condyle were used to assess the cell response and bone ingrowth, respectively. Ta(2)O(5) coatings and Mg-incorporating Ta(2)O(5) coatings were both homogeneously deposited on porous scaffolds. In vitro studies revealed that both coatings exhibit enhanced cell proliferation, ALP activity, osteogenic gene expression and mineralization compared with the uncoated Ti6Al4V scaffold. Especially for Mg-incorporating Ta(2)O(5) coatings, great improvements were observed. In vivo studies, including radiographic examination, fluorochrome labeling and histological evaluation also followed similar trends. Also, bone ingrowth to scaffolds with Mg-incorporating Ta(2)O(5) coatings exhibited the most significant increase compared with uncoated and Ta(2)O(5) coated scaffolds. All the above results indicate that Mg-doped Ta(2)O(5) coatings are an effective tool for facilitating osteointegration of conventional porous Ti6Al4V scaffolds. |
format | Online Article Text |
id | pubmed-10632765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-106327652023-11-10 Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating Zhang, Bo Feng, Jun Chen, Shuo Liao, Ruohan Zhang, Chengdong Luo, Xuwei Yang, Zelong Xiao, Dongqin He, Kui Duan, Ke RSC Adv Chemistry In recent years, additive manufacturing techniques have been used to fabricate 3D titanium (Ti)-based scaffolds for production of desirable complex shapes. However, insufficient osteointegration of porous Ti-based scaffolds can elicit long-term complications (e.g., aseptic loosening) and need further revision surgery. In this study, a magnesium (Mg)-incorporating tantalum (Ta) coating was deposited on a 3D Ti6Al4V scaffold using a sol–gel method for enhancing its osteogenic properties. To evaluate the biofunction of this surface, bone mesenchymal stem cells and rabbit femoral condyle were used to assess the cell response and bone ingrowth, respectively. Ta(2)O(5) coatings and Mg-incorporating Ta(2)O(5) coatings were both homogeneously deposited on porous scaffolds. In vitro studies revealed that both coatings exhibit enhanced cell proliferation, ALP activity, osteogenic gene expression and mineralization compared with the uncoated Ti6Al4V scaffold. Especially for Mg-incorporating Ta(2)O(5) coatings, great improvements were observed. In vivo studies, including radiographic examination, fluorochrome labeling and histological evaluation also followed similar trends. Also, bone ingrowth to scaffolds with Mg-incorporating Ta(2)O(5) coatings exhibited the most significant increase compared with uncoated and Ta(2)O(5) coated scaffolds. All the above results indicate that Mg-doped Ta(2)O(5) coatings are an effective tool for facilitating osteointegration of conventional porous Ti6Al4V scaffolds. The Royal Society of Chemistry 2023-11-09 /pmc/articles/PMC10632765/ /pubmed/37954425 http://dx.doi.org/10.1039/d3ra05814j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Zhang, Bo Feng, Jun Chen, Shuo Liao, Ruohan Zhang, Chengdong Luo, Xuwei Yang, Zelong Xiao, Dongqin He, Kui Duan, Ke Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating |
title | Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating |
title_full | Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating |
title_fullStr | Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating |
title_full_unstemmed | Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating |
title_short | Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol–gel Ta(2)O(5) coating |
title_sort | cell response and bone ingrowth to 3d printed ti6al4v scaffolds with mg-incorporating sol–gel ta(2)o(5) coating |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632765/ https://www.ncbi.nlm.nih.gov/pubmed/37954425 http://dx.doi.org/10.1039/d3ra05814j |
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