Cargando…
Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia
OBJECTIVE(S): The prognosis of endometrial cancer (EC) is significantly affected by tumor infiltration and metastasis. Cortactin (CTTN) regulates infiltration and metastasis in other tumors. Studies on the role and mechanism of CTTN in EC are limited and further studies are needed. MATERIALS AND MET...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634056/ https://www.ncbi.nlm.nih.gov/pubmed/37970440 http://dx.doi.org/10.22038/IJBMS.2023.67633.14863 |
_version_ | 1785146189580075008 |
---|---|
author | Lin, Huisi Fan, Yujuan Zhi, Zhifu Pang, Lihong Sun, Dan |
author_facet | Lin, Huisi Fan, Yujuan Zhi, Zhifu Pang, Lihong Sun, Dan |
author_sort | Lin, Huisi |
collection | PubMed |
description | OBJECTIVE(S): The prognosis of endometrial cancer (EC) is significantly affected by tumor infiltration and metastasis. Cortactin (CTTN) regulates infiltration and metastasis in other tumors. Studies on the role and mechanism of CTTN in EC are limited and further studies are needed. MATERIALS AND METHODS: Quantitative PCR and immunohistochemistry were used to detect Ras-associated C3 botulinum toxin substrate 1 (Rac1) and CTTN in EC and normal tissues. The relationship between the expression of these two genes and their prognostic factors was analyzed. A CTTN-RNAi lentiviral system was constructed and transfected into EC cells. Migration and invasion were evaluated by scratch assay, transwell migration, and invasion assays. Pseudopodia formation was observed by immunofluorescence staining. Western blotting was performed to detect the expression of Rac1. RESULTS: The expression levels of Rac1 and CTTN in EC tissues were significantly higher than those in normal tissues. In the EC group, Rac1 and CTTN levels were correlated. The protein expression levels of Rac1 and CTTN were related to myometrial invasion and stage. After CTTN knockdown, the migration rate, invasiveness, and migratory ability of EC cells decreased significantly. Lamellipodia was observed to disappear with the appearance of blebs. Rac1 protein expression was decreased after CTTN knockdown. CONCLUSION: CTTN may promote the invasion and migration of EC by lamellipodia. This effect may be related to the regulation of Rac1 by CTTN. |
format | Online Article Text |
id | pubmed-10634056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-106340562023-11-15 Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia Lin, Huisi Fan, Yujuan Zhi, Zhifu Pang, Lihong Sun, Dan Iran J Basic Med Sci Original Article OBJECTIVE(S): The prognosis of endometrial cancer (EC) is significantly affected by tumor infiltration and metastasis. Cortactin (CTTN) regulates infiltration and metastasis in other tumors. Studies on the role and mechanism of CTTN in EC are limited and further studies are needed. MATERIALS AND METHODS: Quantitative PCR and immunohistochemistry were used to detect Ras-associated C3 botulinum toxin substrate 1 (Rac1) and CTTN in EC and normal tissues. The relationship between the expression of these two genes and their prognostic factors was analyzed. A CTTN-RNAi lentiviral system was constructed and transfected into EC cells. Migration and invasion were evaluated by scratch assay, transwell migration, and invasion assays. Pseudopodia formation was observed by immunofluorescence staining. Western blotting was performed to detect the expression of Rac1. RESULTS: The expression levels of Rac1 and CTTN in EC tissues were significantly higher than those in normal tissues. In the EC group, Rac1 and CTTN levels were correlated. The protein expression levels of Rac1 and CTTN were related to myometrial invasion and stage. After CTTN knockdown, the migration rate, invasiveness, and migratory ability of EC cells decreased significantly. Lamellipodia was observed to disappear with the appearance of blebs. Rac1 protein expression was decreased after CTTN knockdown. CONCLUSION: CTTN may promote the invasion and migration of EC by lamellipodia. This effect may be related to the regulation of Rac1 by CTTN. Mashhad University of Medical Sciences 2023 /pmc/articles/PMC10634056/ /pubmed/37970440 http://dx.doi.org/10.22038/IJBMS.2023.67633.14863 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lin, Huisi Fan, Yujuan Zhi, Zhifu Pang, Lihong Sun, Dan Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
title | Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
title_full | Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
title_fullStr | Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
title_full_unstemmed | Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
title_short | Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
title_sort | short-hairpin rna-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634056/ https://www.ncbi.nlm.nih.gov/pubmed/37970440 http://dx.doi.org/10.22038/IJBMS.2023.67633.14863 |
work_keys_str_mv | AT linhuisi shorthairpinrnamediatedsuppressionofcortactinmayinhibitthemigrationandinvasionabilitiesofendometrialcancercellsbyreducinglamellipodia AT fanyujuan shorthairpinrnamediatedsuppressionofcortactinmayinhibitthemigrationandinvasionabilitiesofendometrialcancercellsbyreducinglamellipodia AT zhizhifu shorthairpinrnamediatedsuppressionofcortactinmayinhibitthemigrationandinvasionabilitiesofendometrialcancercellsbyreducinglamellipodia AT panglihong shorthairpinrnamediatedsuppressionofcortactinmayinhibitthemigrationandinvasionabilitiesofendometrialcancercellsbyreducinglamellipodia AT sundan shorthairpinrnamediatedsuppressionofcortactinmayinhibitthemigrationandinvasionabilitiesofendometrialcancercellsbyreducinglamellipodia |