Cargando…
The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers
BACKGROUND: The investigation of the presence of extended-spectrum beta-lactamase (ESBL) within Enterobacteriaceae in both fecal carriers and patients is an essential matter. Furthermore, the assessment of distinct characteristics exhibited by resistant bacteria obtained from fecal carriers and pati...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634096/ https://www.ncbi.nlm.nih.gov/pubmed/37940865 http://dx.doi.org/10.1186/s12879-023-08746-3 |
_version_ | 1785132758045032448 |
---|---|
author | Aghamohammad, Shadi Shahcheraghi, Fereshteh |
author_facet | Aghamohammad, Shadi Shahcheraghi, Fereshteh |
author_sort | Aghamohammad, Shadi |
collection | PubMed |
description | BACKGROUND: The investigation of the presence of extended-spectrum beta-lactamase (ESBL) within Enterobacteriaceae in both fecal carriers and patients is an essential matter. Furthermore, the assessment of distinct characteristics exhibited by resistant bacteria obtained from fecal carriers and patients, as well as the comparison of these characteristics between the two groups, could provide a deeper understanding of how the resistant isolates can remain concealed within a dormant reservoir and intensify antimicrobial resistance. The aim of the present study was to concentrate on the comparison of the antimicrobial resistance pattern and molecular features between strains obtained from clinical and carrier sources. MATERIAL AND METHODS: A total of 142 clinical samples and 120 rectal swabs were collected from June to October 2016. ESBL screening was performed using the double-disk synergy test. PCR was done for the detection of ESBL genes. Assessment of biofilm formation, virulence factor genes, and MLVA was performed for K. pneumonae isolates. Phylogroup typing was performed for E. coli isolates. RESULTS: Of 146 samples, 67.6% were E. coli, and 32.4% were K. pneumoniae. The rate of bla(CTXM-15) was 89.4%. In K. pneumoniae type D, ompk35 and fimH were the highest. All the K. pneumoniae isolates were classified into 12 mini clusters and the clinical isolates were characterized into 7 mini clusters. The phylogroup B2 in ESBL-EC was the highest (56.2%). DISCUSSION: Comparison of molecular characteristics and clonal relatedness between fecal carriers and patients showed noticeable relatedness and similarity which may indicate that ESBL-KP can be colonized with the same profiles in different settings and, therefore, may be widely distributed in both community and hospital settings. Therefore, implementation of control protocols, including surveillance of the fecal carriers, could impressively reduce silent reservoirs without clinical symptoms as well as patient rates. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-023-08746-3. |
format | Online Article Text |
id | pubmed-10634096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-106340962023-11-10 The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers Aghamohammad, Shadi Shahcheraghi, Fereshteh BMC Infect Dis Research BACKGROUND: The investigation of the presence of extended-spectrum beta-lactamase (ESBL) within Enterobacteriaceae in both fecal carriers and patients is an essential matter. Furthermore, the assessment of distinct characteristics exhibited by resistant bacteria obtained from fecal carriers and patients, as well as the comparison of these characteristics between the two groups, could provide a deeper understanding of how the resistant isolates can remain concealed within a dormant reservoir and intensify antimicrobial resistance. The aim of the present study was to concentrate on the comparison of the antimicrobial resistance pattern and molecular features between strains obtained from clinical and carrier sources. MATERIAL AND METHODS: A total of 142 clinical samples and 120 rectal swabs were collected from June to October 2016. ESBL screening was performed using the double-disk synergy test. PCR was done for the detection of ESBL genes. Assessment of biofilm formation, virulence factor genes, and MLVA was performed for K. pneumonae isolates. Phylogroup typing was performed for E. coli isolates. RESULTS: Of 146 samples, 67.6% were E. coli, and 32.4% were K. pneumoniae. The rate of bla(CTXM-15) was 89.4%. In K. pneumoniae type D, ompk35 and fimH were the highest. All the K. pneumoniae isolates were classified into 12 mini clusters and the clinical isolates were characterized into 7 mini clusters. The phylogroup B2 in ESBL-EC was the highest (56.2%). DISCUSSION: Comparison of molecular characteristics and clonal relatedness between fecal carriers and patients showed noticeable relatedness and similarity which may indicate that ESBL-KP can be colonized with the same profiles in different settings and, therefore, may be widely distributed in both community and hospital settings. Therefore, implementation of control protocols, including surveillance of the fecal carriers, could impressively reduce silent reservoirs without clinical symptoms as well as patient rates. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-023-08746-3. BioMed Central 2023-11-08 /pmc/articles/PMC10634096/ /pubmed/37940865 http://dx.doi.org/10.1186/s12879-023-08746-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Aghamohammad, Shadi Shahcheraghi, Fereshteh The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers |
title | The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers |
title_full | The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers |
title_fullStr | The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers |
title_full_unstemmed | The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers |
title_short | The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers |
title_sort | notable relatedness between esbl producing enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634096/ https://www.ncbi.nlm.nih.gov/pubmed/37940865 http://dx.doi.org/10.1186/s12879-023-08746-3 |
work_keys_str_mv | AT aghamohammadshadi thenotablerelatednessbetweenesblproducingenterobacteriaceaeisolatedfromclinicalsamplesandasymptomaticfecalcarriers AT shahcheraghifereshteh thenotablerelatednessbetweenesblproducingenterobacteriaceaeisolatedfromclinicalsamplesandasymptomaticfecalcarriers AT aghamohammadshadi notablerelatednessbetweenesblproducingenterobacteriaceaeisolatedfromclinicalsamplesandasymptomaticfecalcarriers AT shahcheraghifereshteh notablerelatednessbetweenesblproducingenterobacteriaceaeisolatedfromclinicalsamplesandasymptomaticfecalcarriers |