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Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution

[Image: see text] This study investigated the conjugation of chitosan with the insulin-mimetic [meso-tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−), VO(tpps), in an aqueous medium as a function of conjugation time, VO(tpps) concentrations, and temperatures. To validate the synthesis of...

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Autores principales: Shaha, Chironjit Kumar, Sarker, Bithy, Mahalanobish, Shurid Kishore, Hossain, Md. Sharif, Karmaker, Subarna, Saha, Tapan Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634234/
https://www.ncbi.nlm.nih.gov/pubmed/37970023
http://dx.doi.org/10.1021/acsomega.3c05804
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author Shaha, Chironjit Kumar
Sarker, Bithy
Mahalanobish, Shurid Kishore
Hossain, Md. Sharif
Karmaker, Subarna
Saha, Tapan Kumar
author_facet Shaha, Chironjit Kumar
Sarker, Bithy
Mahalanobish, Shurid Kishore
Hossain, Md. Sharif
Karmaker, Subarna
Saha, Tapan Kumar
author_sort Shaha, Chironjit Kumar
collection PubMed
description [Image: see text] This study investigated the conjugation of chitosan with the insulin-mimetic [meso-tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−), VO(tpps), in an aqueous medium as a function of conjugation time, VO(tpps) concentrations, and temperatures. To validate the synthesis of chitosan-VO(tpps) conjugate, UV–visible and Fourier transform infrared spectrophotometric techniques were utilized. Conjugate formation is ascribed to the electrostatic interaction between the NH(3)(+) units of chitosan and the SO(3)(–) units of VO(tpps). Chitosan enhances the stability of VO(tpps) in an aqueous medium (pH 2.5). VO(tpps) conjugation with chitosan was best explained by pseudo-second-order kinetic and Langmuir isotherm models based on kinetic and isotherm studies. The Langmuir equation determined that the maximal ability of VO(tpps) conjugated with each gram of chitosan was 39.22 μmol at a solution temperature of 45 °C. Activation energy and thermodynamic studies (E(a): 8.78 kJ/mol, ΔG: −24.52 to −27.55 kJ/mol, ΔS: 204.22 J/(mol K), and ΔH: 37.30 kJ/mol) reveal that conjugation is endothermic and physical in nature. The discharge of VO(tpps) from conjugate was analyzed in freshly prepared 0.1 mol/L phosphate buffer (pH 7.4) at 37 °C. The release of VO(tpps) from the conjugate is a two-phase process best explained by the Higuchi model, according to a kinetic analysis of the release data. Taking into consideration all experimental findings, it is proposed that chitosan can be used to formulate both solid and liquid insulin-mimetic chitosan-VO(tpps) conjugates.
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spelling pubmed-106342342023-11-15 Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution Shaha, Chironjit Kumar Sarker, Bithy Mahalanobish, Shurid Kishore Hossain, Md. Sharif Karmaker, Subarna Saha, Tapan Kumar ACS Omega [Image: see text] This study investigated the conjugation of chitosan with the insulin-mimetic [meso-tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−), VO(tpps), in an aqueous medium as a function of conjugation time, VO(tpps) concentrations, and temperatures. To validate the synthesis of chitosan-VO(tpps) conjugate, UV–visible and Fourier transform infrared spectrophotometric techniques were utilized. Conjugate formation is ascribed to the electrostatic interaction between the NH(3)(+) units of chitosan and the SO(3)(–) units of VO(tpps). Chitosan enhances the stability of VO(tpps) in an aqueous medium (pH 2.5). VO(tpps) conjugation with chitosan was best explained by pseudo-second-order kinetic and Langmuir isotherm models based on kinetic and isotherm studies. The Langmuir equation determined that the maximal ability of VO(tpps) conjugated with each gram of chitosan was 39.22 μmol at a solution temperature of 45 °C. Activation energy and thermodynamic studies (E(a): 8.78 kJ/mol, ΔG: −24.52 to −27.55 kJ/mol, ΔS: 204.22 J/(mol K), and ΔH: 37.30 kJ/mol) reveal that conjugation is endothermic and physical in nature. The discharge of VO(tpps) from conjugate was analyzed in freshly prepared 0.1 mol/L phosphate buffer (pH 7.4) at 37 °C. The release of VO(tpps) from the conjugate is a two-phase process best explained by the Higuchi model, according to a kinetic analysis of the release data. Taking into consideration all experimental findings, it is proposed that chitosan can be used to formulate both solid and liquid insulin-mimetic chitosan-VO(tpps) conjugates. American Chemical Society 2023-10-24 /pmc/articles/PMC10634234/ /pubmed/37970023 http://dx.doi.org/10.1021/acsomega.3c05804 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Shaha, Chironjit Kumar
Sarker, Bithy
Mahalanobish, Shurid Kishore
Hossain, Md. Sharif
Karmaker, Subarna
Saha, Tapan Kumar
Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution
title Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution
title_full Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution
title_fullStr Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution
title_full_unstemmed Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution
title_short Kinetics, Equilibrium, and Thermodynamics for Conjugation of Chitosan with Insulin-Mimetic [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4−) in an Aqueous Solution
title_sort kinetics, equilibrium, and thermodynamics for conjugation of chitosan with insulin-mimetic [meso-tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(iv)(4−) in an aqueous solution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634234/
https://www.ncbi.nlm.nih.gov/pubmed/37970023
http://dx.doi.org/10.1021/acsomega.3c05804
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