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Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus
Bacteria use population heterogeneity, the presence of more than one phenotypic variant in a clonal population, to endure diverse environmental challenges – a ‘bet-hedging’ strategy. Phenotypic variants have been described in many bacteria, but the phenomenon is not well-understood in mycobacteria,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634367/ https://www.ncbi.nlm.nih.gov/pubmed/37862100 http://dx.doi.org/10.1099/mic.0.001402 |
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author | Born, Sarah E. M. Reichlen, Matthew J. Bartek, Iona L. Benoit, Jeanne B. Frank, Daniel N. Voskuil, Martin I. |
author_facet | Born, Sarah E. M. Reichlen, Matthew J. Bartek, Iona L. Benoit, Jeanne B. Frank, Daniel N. Voskuil, Martin I. |
author_sort | Born, Sarah E. M. |
collection | PubMed |
description | Bacteria use population heterogeneity, the presence of more than one phenotypic variant in a clonal population, to endure diverse environmental challenges – a ‘bet-hedging’ strategy. Phenotypic variants have been described in many bacteria, but the phenomenon is not well-understood in mycobacteria, including the environmental factors that influence heterogeneity. Here, we describe three reproducible morphological variants in M. smegmatis – smooth, rough, and an intermediate morphotype that predominated under typical laboratory conditions. M. abscessus has two recognized morphotypes, smooth and rough. Interestingly, M. tuberculosis exists in only a rough form. The shift from smooth to rough in both M. smegmatis and M. abscessus was observed over time in extended static culture, however the frequency of the rough morphotype was high in pellicle preparations compared to planktonic culture, suggesting a role for an aggregated microenvironment in the shift to the rough form. Differences in growth rate, biofilm formation, cell wall composition, and drug tolerance were noted among M. smegmatis and M. abscessus variants. Deletion of the global regulator lsr2 shifted the M. smegmatis intermediate morphotype to a smooth form but did not fully phenocopy the naturally generated smooth morphotype, indicating Lsr2 is likely downstream of the initiating regulatory cascade that controls these morphotypes. Rough forms typically correlate with higher invasiveness and worse outcomes during infection and our findings indicate the shift to this rough form is promoted by aggregation. Our findings suggest that mycobacterial population heterogeneity, reflected in colony morphotypes, is a reproducible, programmed phenomenon that plays a role in adaptation to unique environments and this heterogeneity may influence infection progression and response to treatment. |
format | Online Article Text |
id | pubmed-10634367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-106343672023-11-15 Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus Born, Sarah E. M. Reichlen, Matthew J. Bartek, Iona L. Benoit, Jeanne B. Frank, Daniel N. Voskuil, Martin I. Microbiology (Reading) Microbial Physiology, Biochemistry and Metabolism Bacteria use population heterogeneity, the presence of more than one phenotypic variant in a clonal population, to endure diverse environmental challenges – a ‘bet-hedging’ strategy. Phenotypic variants have been described in many bacteria, but the phenomenon is not well-understood in mycobacteria, including the environmental factors that influence heterogeneity. Here, we describe three reproducible morphological variants in M. smegmatis – smooth, rough, and an intermediate morphotype that predominated under typical laboratory conditions. M. abscessus has two recognized morphotypes, smooth and rough. Interestingly, M. tuberculosis exists in only a rough form. The shift from smooth to rough in both M. smegmatis and M. abscessus was observed over time in extended static culture, however the frequency of the rough morphotype was high in pellicle preparations compared to planktonic culture, suggesting a role for an aggregated microenvironment in the shift to the rough form. Differences in growth rate, biofilm formation, cell wall composition, and drug tolerance were noted among M. smegmatis and M. abscessus variants. Deletion of the global regulator lsr2 shifted the M. smegmatis intermediate morphotype to a smooth form but did not fully phenocopy the naturally generated smooth morphotype, indicating Lsr2 is likely downstream of the initiating regulatory cascade that controls these morphotypes. Rough forms typically correlate with higher invasiveness and worse outcomes during infection and our findings indicate the shift to this rough form is promoted by aggregation. Our findings suggest that mycobacterial population heterogeneity, reflected in colony morphotypes, is a reproducible, programmed phenomenon that plays a role in adaptation to unique environments and this heterogeneity may influence infection progression and response to treatment. Microbiology Society 2023-10-20 /pmc/articles/PMC10634367/ /pubmed/37862100 http://dx.doi.org/10.1099/mic.0.001402 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Microbial Physiology, Biochemistry and Metabolism Born, Sarah E. M. Reichlen, Matthew J. Bartek, Iona L. Benoit, Jeanne B. Frank, Daniel N. Voskuil, Martin I. Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus |
title | Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus
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title_full | Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus
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title_fullStr | Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus
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title_full_unstemmed | Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus
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title_short | Population heterogeneity in Mycobacterium smegmatis and Mycobacterium abscessus
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title_sort | population heterogeneity in mycobacterium smegmatis and mycobacterium abscessus |
topic | Microbial Physiology, Biochemistry and Metabolism |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634367/ https://www.ncbi.nlm.nih.gov/pubmed/37862100 http://dx.doi.org/10.1099/mic.0.001402 |
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