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In vivo affinity maturation of murine B cells reprogrammed to express human antibodies

CRISPR-edited murine B cells engineered to express human antibody variable chains proliferate, class switch, and secrete these antibodies in vaccinated mice. However, current strategies disrupt the heavy-chain locus, resulting in inefficient somatic hypermutation without functional affinity maturati...

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Detalles Bibliográficos
Autores principales:	Yin, Yiming, Guo, Yan, Jiang, Yuxuan, Quinlan, Brian, Peng, Haiyong, Crynen, Gogce, He, Wenhui, Zhang, Lizhou, Ou, Tianling, Bailey, Charles C., Farzan, Michael
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634681/ https://www.ncbi.nlm.nih.gov/pubmed/37961481 http://dx.doi.org/10.1101/2023.10.20.563154

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