Cargando…

Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study

Small extracellular vesicles (sEVs) are heterogeneous biological vesicles released by cells under both physiological and pathological conditions. Due to their potential as valuable diagnostic and prognostic biomarkers in human blood, there is a pressing need to develop effective methods for isolatin...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kong, Fang, Upadya, Megha, Wong, Andrew See Weng, Dalan, Rinkoo, Dao, Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634961/ https://www.ncbi.nlm.nih.gov/pubmed/37961562 http://dx.doi.org/10.1101/2023.10.30.564707

Ejemplares similares

Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles
por: Wei, Rui, et al.
Publicado: (2020)

Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential
por: Takov, Kaloyan, et al.
Publicado: (2018)

Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles
por: Visan, Kekoolani S., et al.
Publicado: (2022)

Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation
por: Yuana, Yuana, et al.
Publicado: (2014)

Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus Ultracentrifugation
por: Soares, Maria, et al.
Publicado: (2023)

Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations
por: Iwai, Kazuya, et al.
Publicado: (2016)

Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma
por: Onódi, Zsófia, et al.
Publicado: (2018)

Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
por: Duong, Phat, et al.
Publicado: (2019)

Correction: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
por: Duong, Phat, et al.
Publicado: (2020)

Isolation of Hepatic and Adipose-Tissue-Derived Extracellular Vesicles Using Density Gradient Separation and Size Exclusion Chromatography
por: Martínez-Greene, Juan Alfonso, et al.
Publicado: (2023)

Enrichment of calcifying extracellular vesicles using density-based ultracentrifugation protocol
por: Hutcheson, Joshua D., et al.
Publicado: (2014)

Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation
por: Arab, T, et al.
Publicado: (2019)

Extracellular Vesicles from Follicular and Ampullary Fluid Isolated by Density Gradient Ultracentrifugation Improve Bovine Embryo Development and Quality
por: Asaadi, Anise, et al.
Publicado: (2021)

Comparison of High-Performance Liquid Chromatography with Sucrose Density Gradient Ultracentrifugation for the Quantification of Foot-and-Mouth Disease Vaccine Antigens
por: Kim, Ah-Young, et al.
Publicado: (2022)

Isolation of Small Extracellular Vesicles From Human Serum Using a Combination of Ultracentrifugation With Polymer-Based Precipitation
por: Ryu, Kyung Ju, et al.
Publicado: (2020)

Investigation of procoagulant activity in extracellular vesicles isolated by differential ultracentrifugation
por: Nielsen, Thøger, et al.
Publicado: (2018)

Purification of human respiratory syncytial virus by ultracentrifugation in iodixanol density gradient
por: Gias, E., et al.
Publicado: (2008)

Single-step isolation of extracellular vesicles by size-exclusion chromatography
por: Böing, Anita N., et al.
Publicado: (2014)

Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods
por: Baranyai, Tamás, et al.
Publicado: (2015)

Characterisation of mass distributions of solvent-fractionated lignins using analytical ultracentrifugation and size exclusion chromatography methods
por: Lu, Yudong, et al.
Publicado: (2021)

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
por: Kaddour, Hussein, et al.
Publicado: (2021)

Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS
por: Alameldin, Sara, et al.
Publicado: (2021)

Optimizing high-throughput viral vector characterization with density gradient equilibrium analytical ultracentrifugation
por: Sternisha, Shawn M., et al.
Publicado: (2023)

Protocol for the separation of extracellular vesicles by ultracentrifugation from in vitro cell culture models
por: Chhoy, Peter, et al.
Publicado: (2021)

Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales
por: Ludwig, Anna-Kristin, et al.
Publicado: (2018)

Size-exclusion chromatography-based enrichment of extracellular vesicles from urine samples
por: Lozano-Ramos, Inés, et al.
Publicado: (2015)

Size-Exclusion Chromatography as a Technique for the Investigation of Novel Extracellular Vesicles in Cancer
por: Liu, Daniel S. K., et al.
Publicado: (2020)

High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
por: Bellotti, Cristina, et al.
Publicado: (2021)

Residual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment
por: Musante, Luca, et al.
Publicado: (2016)

Analysis of RNA yield in extracellular vesicles isolated by membrane affinity column and differential ultracentrifugation
por: Gutiérrez García, Gilberto, et al.
Publicado: (2020)

Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography
por: Foers, Andrew D., et al.
Publicado: (2018)

Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography
por: Blans, Kristine, et al.
Publicado: (2017)

Detection and phenotyping of extracellular vesicles by size exclusion chromatography coupled with on-line fluorescence detection
por: Kitka, Diána, et al.
Publicado: (2019)

Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma
por: Yang, Yang, et al.
Publicado: (2021)

Isolation of HDL by sequential flotation ultracentrifugation followed by size exclusion chromatography reveals size-based enrichment of HDL-associated proteins
por: Zheng, Jack Jingyuan, et al.
Publicado: (2021)

Large Aggregates Are the Major Soluble Aβ Species in AD Brain Fractionated with Density Gradient Ultracentrifugation
por: Sehlin, Dag, et al.
Publicado: (2012)

Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation
por: Mukhopadhya, Anindya, et al.
Publicado: (2021)

Metabolomics of small extracellular vesicles derived from isocitrate dehydrogenase 1-mutant HCT116 cells collected by semi-automated size exclusion chromatography
por: Hayasaka, Ryosuke, et al.
Publicado: (2023)

Efficient extracellular vesicle isolation by combining cell media modifications, ultrafiltration, and size-exclusion chromatography
por: Guerreiro, Eduarda M., et al.
Publicado: (2018)

Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography
por: Corso, Giulia, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_384j74bugdsa472im7huprqed6