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Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies

INTRODUCTION: The apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts. METHODS: To advance ou...

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Autores principales: Cruz-Bustos, Teresa, Dolezal, Marlies, Feix, Anna Sophia, Ruttkowski, Bärbel, Hummel, Karin, Razzazi-Fazeli, Ebrahim, Joachim, Anja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635411/
https://www.ncbi.nlm.nih.gov/pubmed/37953800
http://dx.doi.org/10.3389/fcimb.2023.1271731
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author Cruz-Bustos, Teresa
Dolezal, Marlies
Feix, Anna Sophia
Ruttkowski, Bärbel
Hummel, Karin
Razzazi-Fazeli, Ebrahim
Joachim, Anja
author_facet Cruz-Bustos, Teresa
Dolezal, Marlies
Feix, Anna Sophia
Ruttkowski, Bärbel
Hummel, Karin
Razzazi-Fazeli, Ebrahim
Joachim, Anja
author_sort Cruz-Bustos, Teresa
collection PubMed
description INTRODUCTION: The apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts. METHODS: To advance our understanding of the parasite’s cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis. RESULTS: A total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production. DISCUSSION: These findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages.
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spelling pubmed-106354112023-11-10 Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies Cruz-Bustos, Teresa Dolezal, Marlies Feix, Anna Sophia Ruttkowski, Bärbel Hummel, Karin Razzazi-Fazeli, Ebrahim Joachim, Anja Front Cell Infect Microbiol Cellular and Infection Microbiology INTRODUCTION: The apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts. METHODS: To advance our understanding of the parasite’s cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis. RESULTS: A total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production. DISCUSSION: These findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages. Frontiers Media S.A. 2023-10-25 /pmc/articles/PMC10635411/ /pubmed/37953800 http://dx.doi.org/10.3389/fcimb.2023.1271731 Text en Copyright © 2023 Cruz-Bustos, Dolezal, Feix, Ruttkowski, Hummel, Razzazi-Fazeli and Joachim https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Cruz-Bustos, Teresa
Dolezal, Marlies
Feix, Anna Sophia
Ruttkowski, Bärbel
Hummel, Karin
Razzazi-Fazeli, Ebrahim
Joachim, Anja
Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies
title Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies
title_full Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies
title_fullStr Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies
title_full_unstemmed Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies
title_short Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies
title_sort unravelling the sexual developmental biology of cystoisospora suis, a model for comparative coccidian parasite studies
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635411/
https://www.ncbi.nlm.nih.gov/pubmed/37953800
http://dx.doi.org/10.3389/fcimb.2023.1271731
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