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Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach

One of the most prevalent chronic conditions affecting older men is benign prostatic hyperplasia (BPH), causing severe annoyance and embarrassment to patients. The pathogenesis of BPH has been connected to epithelial proliferation, inflammation, deranged redox balance, and apoptosis. Diacerein (DIA)...

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Autores principales: Rasheed, Rabab Ahmed, Sadek, A. S., Khattab, R. T., Elkhamisy, Fatma Alzahraa A., Abdelfattah, Heba Abdelrazak, Elshaer, Mohamed M. A., Almutairi, Saeedah Musaed, Hussein, Dina S., Embaby, Azza Saleh, Almoatasem, Mai A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635502/
https://www.ncbi.nlm.nih.gov/pubmed/37943844
http://dx.doi.org/10.1371/journal.pone.0293682
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author Rasheed, Rabab Ahmed
Sadek, A. S.
Khattab, R. T.
Elkhamisy, Fatma Alzahraa A.
Abdelfattah, Heba Abdelrazak
Elshaer, Mohamed M. A.
Almutairi, Saeedah Musaed
Hussein, Dina S.
Embaby, Azza Saleh
Almoatasem, Mai A. M.
author_facet Rasheed, Rabab Ahmed
Sadek, A. S.
Khattab, R. T.
Elkhamisy, Fatma Alzahraa A.
Abdelfattah, Heba Abdelrazak
Elshaer, Mohamed M. A.
Almutairi, Saeedah Musaed
Hussein, Dina S.
Embaby, Azza Saleh
Almoatasem, Mai A. M.
author_sort Rasheed, Rabab Ahmed
collection PubMed
description One of the most prevalent chronic conditions affecting older men is benign prostatic hyperplasia (BPH), causing severe annoyance and embarrassment to patients. The pathogenesis of BPH has been connected to epithelial proliferation, inflammation, deranged redox balance, and apoptosis. Diacerein (DIA), the anthraquinone derivative, is a non-steroidal anti-inflammatory drug. This study intended to investigate the ameliorative effect of DIA on the prostatic histology in testosterone-induced BPH in rats. BPH was experimentally induced by daily subcutaneous injection of testosterone propionate for four weeks. The treated group received DIA daily for a further two weeks after induction of BPH. Rats’ body and prostate weights, serum-free testosterone, dihydrotestosterone, and PSA were evaluated. Prostatic tissue was processed for measuring redox balance and histopathological examination. The BPH group had increased body and prostate weights, serum testosterone, dihydrotestosterone, PSA, and oxidative stress. Histologically, there were marked acinar epithelial and stromal hyperplasia, inflammatory infiltrates, and increased collagen deposition. An immunohistochemical study showed an increase in the inflammatory TNF-α and the proliferative PCNA markers. Treatment with DIA markedly decreased the prostate weight and plasma hormones, improved tissue redox balance, repaired the histological changes, and increased the proapoptotic caspase 3 expression besides the substantial reduction in TNF-α and PCNA expression. In conclusion, our study underscored DIA’s potential to alleviate the prostatic hyperplastic and inflammatory changes in BPH through its antioxidant, anti-inflammatory, antiproliferative, and apoptosis-inducing effects, rendering it an effective, innovative treatment for BPH.
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spelling pubmed-106355022023-11-10 Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach Rasheed, Rabab Ahmed Sadek, A. S. Khattab, R. T. Elkhamisy, Fatma Alzahraa A. Abdelfattah, Heba Abdelrazak Elshaer, Mohamed M. A. Almutairi, Saeedah Musaed Hussein, Dina S. Embaby, Azza Saleh Almoatasem, Mai A. M. PLoS One Research Article One of the most prevalent chronic conditions affecting older men is benign prostatic hyperplasia (BPH), causing severe annoyance and embarrassment to patients. The pathogenesis of BPH has been connected to epithelial proliferation, inflammation, deranged redox balance, and apoptosis. Diacerein (DIA), the anthraquinone derivative, is a non-steroidal anti-inflammatory drug. This study intended to investigate the ameliorative effect of DIA on the prostatic histology in testosterone-induced BPH in rats. BPH was experimentally induced by daily subcutaneous injection of testosterone propionate for four weeks. The treated group received DIA daily for a further two weeks after induction of BPH. Rats’ body and prostate weights, serum-free testosterone, dihydrotestosterone, and PSA were evaluated. Prostatic tissue was processed for measuring redox balance and histopathological examination. The BPH group had increased body and prostate weights, serum testosterone, dihydrotestosterone, PSA, and oxidative stress. Histologically, there were marked acinar epithelial and stromal hyperplasia, inflammatory infiltrates, and increased collagen deposition. An immunohistochemical study showed an increase in the inflammatory TNF-α and the proliferative PCNA markers. Treatment with DIA markedly decreased the prostate weight and plasma hormones, improved tissue redox balance, repaired the histological changes, and increased the proapoptotic caspase 3 expression besides the substantial reduction in TNF-α and PCNA expression. In conclusion, our study underscored DIA’s potential to alleviate the prostatic hyperplastic and inflammatory changes in BPH through its antioxidant, anti-inflammatory, antiproliferative, and apoptosis-inducing effects, rendering it an effective, innovative treatment for BPH. Public Library of Science 2023-11-09 /pmc/articles/PMC10635502/ /pubmed/37943844 http://dx.doi.org/10.1371/journal.pone.0293682 Text en © 2023 Rasheed et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rasheed, Rabab Ahmed
Sadek, A. S.
Khattab, R. T.
Elkhamisy, Fatma Alzahraa A.
Abdelfattah, Heba Abdelrazak
Elshaer, Mohamed M. A.
Almutairi, Saeedah Musaed
Hussein, Dina S.
Embaby, Azza Saleh
Almoatasem, Mai A. M.
Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach
title Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach
title_full Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach
title_fullStr Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach
title_full_unstemmed Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach
title_short Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach
title_sort diacerein provokes apoptosis, improves redox balance, and downregulates pcna and tnf-α in a rat model of testosterone-induced benign prostatic hyperplasia: a new non-invasive approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635502/
https://www.ncbi.nlm.nih.gov/pubmed/37943844
http://dx.doi.org/10.1371/journal.pone.0293682
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