Organic fluorescent probes for live-cell super-resolution imaging

The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells, both adva...

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Detalles Bibliográficos
Autores principales: Duan, Xinxin, Zhang, Meng, Zhang, Yu-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Higher Education Press 2023
Materias:
Mini Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635970/
https://www.ncbi.nlm.nih.gov/pubmed/37946039
http://dx.doi.org/10.1007/s12200-023-00090-3
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author Duan, Xinxin
Zhang, Meng
Zhang, Yu-Hui
author_facet Duan, Xinxin
Zhang, Meng
Zhang, Yu-Hui
author_sort Duan, Xinxin
collection PubMed
description The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells, both advanced imaging systems and excellent fluorescent probes are required. Traditional fluorescent probes have good availability, but that is not the case for probes for live-cell super-resolution imaging. In this review, we first introduce the principles of various super-resolution technologies and their probe requirements, then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging, and finally provide a brief conclusion and overview of the future. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-106359702023-11-11 Organic fluorescent probes for live-cell super-resolution imaging Duan, Xinxin Zhang, Meng Zhang, Yu-Hui Front Optoelectron Mini Review The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells, both advanced imaging systems and excellent fluorescent probes are required. Traditional fluorescent probes have good availability, but that is not the case for probes for live-cell super-resolution imaging. In this review, we first introduce the principles of various super-resolution technologies and their probe requirements, then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging, and finally provide a brief conclusion and overview of the future. GRAPHICAL ABSTRACT: [Image: see text] Higher Education Press 2023-11-10 /pmc/articles/PMC10635970/ /pubmed/37946039 http://dx.doi.org/10.1007/s12200-023-00090-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mini Review
Duan, Xinxin
Zhang, Meng
Zhang, Yu-Hui
Organic fluorescent probes for live-cell super-resolution imaging
title Organic fluorescent probes for live-cell super-resolution imaging
title_full Organic fluorescent probes for live-cell super-resolution imaging
title_fullStr Organic fluorescent probes for live-cell super-resolution imaging
title_full_unstemmed Organic fluorescent probes for live-cell super-resolution imaging
title_short Organic fluorescent probes for live-cell super-resolution imaging
title_sort organic fluorescent probes for live-cell super-resolution imaging
topic Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635970/
https://www.ncbi.nlm.nih.gov/pubmed/37946039
http://dx.doi.org/10.1007/s12200-023-00090-3
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AT zhangyuhui organicfluorescentprobesforlivecellsuperresolutionimaging

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