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Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma

VAMP (Vesicle-associated membrane protein)-associated protein B and C (VAPB) has been widely studied in neurodegenerative diseases such as ALS, but little is known about its role in cancer. Medulloblastoma is a common brain malignancy in children and arises from undifferentiated cells during neurona...

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Autores principales: Faria Assoni, Amanda, Giove Mitsugi, Thiago, Wardenaar, René, Oliveira Ferreira, Raiane, Farias Jandrey, Elisa Helena, Machado Novaes, Gabriela, Fonseca de Oliveira Granha, Isabela, Bakker, Petra, Kaid, Carolini, Zatz, Mayana, Foijer, Floris, Keith Okamoto, Oswaldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636017/
https://www.ncbi.nlm.nih.gov/pubmed/37945695
http://dx.doi.org/10.1038/s41598-023-45319-5
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author Faria Assoni, Amanda
Giove Mitsugi, Thiago
Wardenaar, René
Oliveira Ferreira, Raiane
Farias Jandrey, Elisa Helena
Machado Novaes, Gabriela
Fonseca de Oliveira Granha, Isabela
Bakker, Petra
Kaid, Carolini
Zatz, Mayana
Foijer, Floris
Keith Okamoto, Oswaldo
author_facet Faria Assoni, Amanda
Giove Mitsugi, Thiago
Wardenaar, René
Oliveira Ferreira, Raiane
Farias Jandrey, Elisa Helena
Machado Novaes, Gabriela
Fonseca de Oliveira Granha, Isabela
Bakker, Petra
Kaid, Carolini
Zatz, Mayana
Foijer, Floris
Keith Okamoto, Oswaldo
author_sort Faria Assoni, Amanda
collection PubMed
description VAMP (Vesicle-associated membrane protein)-associated protein B and C (VAPB) has been widely studied in neurodegenerative diseases such as ALS, but little is known about its role in cancer. Medulloblastoma is a common brain malignancy in children and arises from undifferentiated cells during neuronal development. Therefore, medulloblastoma is an interesting model to investigate the possible relationship between VAPB and tumorigenesis. Here we demonstrate that high VAPB expression in medulloblastoma correlates with decreased overall patient survival. Consistent with this clinical correlation, we find that VAPB is required for normal proliferation rates of medulloblastoma cells in vitro and in vivo. Knockout of VAPB (VAPB(KO)) delayed cell cycle progression. Furthermore, transcript levels of WNT-related proteins were decreased in the VAPB(KO). We conclude that VAPB is required for proliferation of medulloblastoma cells, thus revealing VAPB as a potential therapeutic target for medulloblastoma treatment.
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spelling pubmed-106360172023-11-11 Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma Faria Assoni, Amanda Giove Mitsugi, Thiago Wardenaar, René Oliveira Ferreira, Raiane Farias Jandrey, Elisa Helena Machado Novaes, Gabriela Fonseca de Oliveira Granha, Isabela Bakker, Petra Kaid, Carolini Zatz, Mayana Foijer, Floris Keith Okamoto, Oswaldo Sci Rep Article VAMP (Vesicle-associated membrane protein)-associated protein B and C (VAPB) has been widely studied in neurodegenerative diseases such as ALS, but little is known about its role in cancer. Medulloblastoma is a common brain malignancy in children and arises from undifferentiated cells during neuronal development. Therefore, medulloblastoma is an interesting model to investigate the possible relationship between VAPB and tumorigenesis. Here we demonstrate that high VAPB expression in medulloblastoma correlates with decreased overall patient survival. Consistent with this clinical correlation, we find that VAPB is required for normal proliferation rates of medulloblastoma cells in vitro and in vivo. Knockout of VAPB (VAPB(KO)) delayed cell cycle progression. Furthermore, transcript levels of WNT-related proteins were decreased in the VAPB(KO). We conclude that VAPB is required for proliferation of medulloblastoma cells, thus revealing VAPB as a potential therapeutic target for medulloblastoma treatment. Nature Publishing Group UK 2023-11-09 /pmc/articles/PMC10636017/ /pubmed/37945695 http://dx.doi.org/10.1038/s41598-023-45319-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Faria Assoni, Amanda
Giove Mitsugi, Thiago
Wardenaar, René
Oliveira Ferreira, Raiane
Farias Jandrey, Elisa Helena
Machado Novaes, Gabriela
Fonseca de Oliveira Granha, Isabela
Bakker, Petra
Kaid, Carolini
Zatz, Mayana
Foijer, Floris
Keith Okamoto, Oswaldo
Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma
title Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma
title_full Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma
title_fullStr Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma
title_full_unstemmed Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma
title_short Neurodegeneration-associated protein VAPB regulates proliferation in medulloblastoma
title_sort neurodegeneration-associated protein vapb regulates proliferation in medulloblastoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636017/
https://www.ncbi.nlm.nih.gov/pubmed/37945695
http://dx.doi.org/10.1038/s41598-023-45319-5
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