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NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing
The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and the vast dynamic range of the plasma proteome. Here we address these challenges with NUcleic acid Linked Immuno-Sandwich Assay (NULISA™), which improves...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636041/ https://www.ncbi.nlm.nih.gov/pubmed/37945559 http://dx.doi.org/10.1038/s41467-023-42834-x |
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author | Feng, Wei Beer, Joanne C. Hao, Qinyu Ariyapala, Ishara S. Sahajan, Aparna Komarov, Andrei Cha, Katie Moua, Mason Qiu, Xiaolei Xu, Xiaomei Iyengar, Shweta Yoshimura, Thu Nagaraj, Rajini Wang, Li Yu, Ming Engel, Kate Zhen, Lucas Xue, Wen Lee, Chen-jung Park, Chan Ho Peng, Cheng Zhang, Kaiyuan Grzybowski, Adrian Hahm, Johnnie Schmidt, Susanne V. Odainic, Alexandru Spitzer, Jasper Buddika, Kasun Kuo, Dwight Fang, Lei Zhang, Bingqing Chen, Steve Latz, Eicke Yin, Yiyuan Luo, Yuling Ma, Xiao-Jun |
author_facet | Feng, Wei Beer, Joanne C. Hao, Qinyu Ariyapala, Ishara S. Sahajan, Aparna Komarov, Andrei Cha, Katie Moua, Mason Qiu, Xiaolei Xu, Xiaomei Iyengar, Shweta Yoshimura, Thu Nagaraj, Rajini Wang, Li Yu, Ming Engel, Kate Zhen, Lucas Xue, Wen Lee, Chen-jung Park, Chan Ho Peng, Cheng Zhang, Kaiyuan Grzybowski, Adrian Hahm, Johnnie Schmidt, Susanne V. Odainic, Alexandru Spitzer, Jasper Buddika, Kasun Kuo, Dwight Fang, Lei Zhang, Bingqing Chen, Steve Latz, Eicke Yin, Yiyuan Luo, Yuling Ma, Xiao-Jun |
author_sort | Feng, Wei |
collection | PubMed |
description | The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and the vast dynamic range of the plasma proteome. Here we address these challenges with NUcleic acid Linked Immuno-Sandwich Assay (NULISA™), which improves the sensitivity of traditional proximity ligation assays by ~10,000-fold to attomolar level, by suppressing assay background via a dual capture and release mechanism built into oligonucleotide-conjugated antibodies. Highly multiplexed quantification of both low- and high-abundance proteins spanning a wide dynamic range is achieved by attenuating signals from abundant targets with unconjugated antibodies and next-generation sequencing of barcoded reporter DNA. A 200-plex NULISA containing 124 cytokines and chemokines and other proteins demonstrates superior sensitivity to a proximity extension assay in detecting biologically important low-abundance biomarkers in patients with autoimmune diseases and COVID-19. Fully automated NULISA makes broad and in-depth proteomic analysis easily accessible for research and diagnostic applications. |
format | Online Article Text |
id | pubmed-10636041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106360412023-11-11 NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing Feng, Wei Beer, Joanne C. Hao, Qinyu Ariyapala, Ishara S. Sahajan, Aparna Komarov, Andrei Cha, Katie Moua, Mason Qiu, Xiaolei Xu, Xiaomei Iyengar, Shweta Yoshimura, Thu Nagaraj, Rajini Wang, Li Yu, Ming Engel, Kate Zhen, Lucas Xue, Wen Lee, Chen-jung Park, Chan Ho Peng, Cheng Zhang, Kaiyuan Grzybowski, Adrian Hahm, Johnnie Schmidt, Susanne V. Odainic, Alexandru Spitzer, Jasper Buddika, Kasun Kuo, Dwight Fang, Lei Zhang, Bingqing Chen, Steve Latz, Eicke Yin, Yiyuan Luo, Yuling Ma, Xiao-Jun Nat Commun Article The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and the vast dynamic range of the plasma proteome. Here we address these challenges with NUcleic acid Linked Immuno-Sandwich Assay (NULISA™), which improves the sensitivity of traditional proximity ligation assays by ~10,000-fold to attomolar level, by suppressing assay background via a dual capture and release mechanism built into oligonucleotide-conjugated antibodies. Highly multiplexed quantification of both low- and high-abundance proteins spanning a wide dynamic range is achieved by attenuating signals from abundant targets with unconjugated antibodies and next-generation sequencing of barcoded reporter DNA. A 200-plex NULISA containing 124 cytokines and chemokines and other proteins demonstrates superior sensitivity to a proximity extension assay in detecting biologically important low-abundance biomarkers in patients with autoimmune diseases and COVID-19. Fully automated NULISA makes broad and in-depth proteomic analysis easily accessible for research and diagnostic applications. Nature Publishing Group UK 2023-11-09 /pmc/articles/PMC10636041/ /pubmed/37945559 http://dx.doi.org/10.1038/s41467-023-42834-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Feng, Wei Beer, Joanne C. Hao, Qinyu Ariyapala, Ishara S. Sahajan, Aparna Komarov, Andrei Cha, Katie Moua, Mason Qiu, Xiaolei Xu, Xiaomei Iyengar, Shweta Yoshimura, Thu Nagaraj, Rajini Wang, Li Yu, Ming Engel, Kate Zhen, Lucas Xue, Wen Lee, Chen-jung Park, Chan Ho Peng, Cheng Zhang, Kaiyuan Grzybowski, Adrian Hahm, Johnnie Schmidt, Susanne V. Odainic, Alexandru Spitzer, Jasper Buddika, Kasun Kuo, Dwight Fang, Lei Zhang, Bingqing Chen, Steve Latz, Eicke Yin, Yiyuan Luo, Yuling Ma, Xiao-Jun NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing |
title | NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing |
title_full | NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing |
title_fullStr | NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing |
title_full_unstemmed | NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing |
title_short | NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing |
title_sort | nulisa: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636041/ https://www.ncbi.nlm.nih.gov/pubmed/37945559 http://dx.doi.org/10.1038/s41467-023-42834-x |
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