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Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model

BACKGROUND/AIMS: Obstructive sleep apnea (OSA) is prevalent in older patients with idiopathic pulmonary fibrosis (IPF); however, it is underrecognized. OSA is characterized by intermittent hypoxia (IH) and sleep fragmentation. In this study, we evaluated the effects of IH in an older mouse model of...

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Autores principales: Lee, Heayon, Kim, In Kyoung, Im, Jeonghyeon, Jin, Bae Suk, Kim, Hwan Hee, Kim, Sei Won, Yeo, Chang Dong, Lee, Sang Haak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association of Internal Medicine 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636542/
https://www.ncbi.nlm.nih.gov/pubmed/37793985
http://dx.doi.org/10.3904/kjim.2023.090
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author Lee, Heayon
Kim, In Kyoung
Im, Jeonghyeon
Jin, Bae Suk
Kim, Hwan Hee
Kim, Sei Won
Yeo, Chang Dong
Lee, Sang Haak
author_facet Lee, Heayon
Kim, In Kyoung
Im, Jeonghyeon
Jin, Bae Suk
Kim, Hwan Hee
Kim, Sei Won
Yeo, Chang Dong
Lee, Sang Haak
author_sort Lee, Heayon
collection PubMed
description BACKGROUND/AIMS: Obstructive sleep apnea (OSA) is prevalent in older patients with idiopathic pulmonary fibrosis (IPF); however, it is underrecognized. OSA is characterized by intermittent hypoxia (IH) and sleep fragmentation. In this study, we evaluated the effects of IH in an older mouse model of bleomycin-induced lung fibrosis. METHODS: Bleomycin-induced mice (C57BL/6, female) were randomly divided into four groups of young vs. old and room air (RA)-exposed vs. IH-exposed. Mice were exposed to RA or IH (20 cycles/h, FiO(2) nadir 7 ± 0.5%, 8 h/day) for four weeks. The mice were sacrificed on day 28, and blood, bronchoalveolar lavage (BAL) fluid, and lung tissue samples were obtained. RESULTS: The bleomycin-induced IH-exposed (EBI) older group showed more severe inflammation, fibrosis, and oxidative stress than the other groups. The levels of inflammatory cytokines in the serum and BAL fluid increased in the EBI group. Hydroxyproline levels in the lung tissue increased markedly in the EBI group. CONCLUSIONS: This study demonstrates the possible harmful impact of OSA in an elderly mouse model of lung fibrosis. This study further suggests that older patients with IPF and OSA may be more of a concern than younger patients with IPF. Further research is required in this area.
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spelling pubmed-106365422023-11-15 Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model Lee, Heayon Kim, In Kyoung Im, Jeonghyeon Jin, Bae Suk Kim, Hwan Hee Kim, Sei Won Yeo, Chang Dong Lee, Sang Haak Korean J Intern Med Original Article BACKGROUND/AIMS: Obstructive sleep apnea (OSA) is prevalent in older patients with idiopathic pulmonary fibrosis (IPF); however, it is underrecognized. OSA is characterized by intermittent hypoxia (IH) and sleep fragmentation. In this study, we evaluated the effects of IH in an older mouse model of bleomycin-induced lung fibrosis. METHODS: Bleomycin-induced mice (C57BL/6, female) were randomly divided into four groups of young vs. old and room air (RA)-exposed vs. IH-exposed. Mice were exposed to RA or IH (20 cycles/h, FiO(2) nadir 7 ± 0.5%, 8 h/day) for four weeks. The mice were sacrificed on day 28, and blood, bronchoalveolar lavage (BAL) fluid, and lung tissue samples were obtained. RESULTS: The bleomycin-induced IH-exposed (EBI) older group showed more severe inflammation, fibrosis, and oxidative stress than the other groups. The levels of inflammatory cytokines in the serum and BAL fluid increased in the EBI group. Hydroxyproline levels in the lung tissue increased markedly in the EBI group. CONCLUSIONS: This study demonstrates the possible harmful impact of OSA in an elderly mouse model of lung fibrosis. This study further suggests that older patients with IPF and OSA may be more of a concern than younger patients with IPF. Further research is required in this area. The Korean Association of Internal Medicine 2023-11 2023-10-05 /pmc/articles/PMC10636542/ /pubmed/37793985 http://dx.doi.org/10.3904/kjim.2023.090 Text en Copyright © 2023 The Korean Association of Internal Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Heayon
Kim, In Kyoung
Im, Jeonghyeon
Jin, Bae Suk
Kim, Hwan Hee
Kim, Sei Won
Yeo, Chang Dong
Lee, Sang Haak
Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model
title Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model
title_full Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model
title_fullStr Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model
title_full_unstemmed Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model
title_short Effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model
title_sort effects of aging on accompanying intermittent hypoxia in a bleomycin-induced pulmonary fibrosis mouse model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636542/
https://www.ncbi.nlm.nih.gov/pubmed/37793985
http://dx.doi.org/10.3904/kjim.2023.090
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