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A Multimodal Scaffold for SDF1 Delivery Improves Cardiac Function in a Rat Subacute Myocardial Infarct Model
[Image: see text] Ischemic heart disease is one of the leading causes of death worldwide. The efficient delivery of therapeutic growth factors could counteract the adverse prognosis of post-myocardial infarction (post-MI). In this study, a collagen hydrogel that is able to load and appropriately del...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636708/ https://www.ncbi.nlm.nih.gov/pubmed/37566441 http://dx.doi.org/10.1021/acsami.3c04245 |
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author | Perez-Estenaga, Iñigo Chevalier, Merari Tumin Peña, Estefania Abizanda, Gloria Alsharabasy, Amir M. Larequi, Eduardo Cilla, Myriam Perez, Marta M. Gurtubay, Jon Garcia-Yebenes Castro, Manuel Prosper, Felipe Pandit, Abhay Pelacho, Beatriz |
author_facet | Perez-Estenaga, Iñigo Chevalier, Merari Tumin Peña, Estefania Abizanda, Gloria Alsharabasy, Amir M. Larequi, Eduardo Cilla, Myriam Perez, Marta M. Gurtubay, Jon Garcia-Yebenes Castro, Manuel Prosper, Felipe Pandit, Abhay Pelacho, Beatriz |
author_sort | Perez-Estenaga, Iñigo |
collection | PubMed |
description | [Image: see text] Ischemic heart disease is one of the leading causes of death worldwide. The efficient delivery of therapeutic growth factors could counteract the adverse prognosis of post-myocardial infarction (post-MI). In this study, a collagen hydrogel that is able to load and appropriately deliver pro-angiogenic stromal cell-derived factor 1 (SDF1) was physically coupled with a compact collagen membrane in order to provide the suture strength required for surgical implantation. This bilayer collagen-on-collagen scaffold (bCS) showed the suitable physicochemical properties that are needed for efficient implantation, and the scaffold was able to deliver therapeutic growth factors after MI. In vitro collagen matrix biodegradation led to a sustained SDF1 release and a lack of cytotoxicity in the relevant cell cultures. In vivo intervention in a rat subacute MI model resulted in the full integration of the scaffold into the heart after implantation and biocompatibility with the tissue, with a prevalence of anti-inflammatory and pro-angiogenic macrophages, as well as evidence of revascularization and improved cardiac function after 60 days. Moreover, the beneficial effect of the released SDF1 on heart remodeling was confirmed by a significant reduction in cardiac tissue stiffness. Our findings demonstrate that this multimodal scaffold is a desirable matrix that can be used as a drug delivery system and a scaffolding material to promote functional recovery after MI. |
format | Online Article Text |
id | pubmed-10636708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-106367082023-11-15 A Multimodal Scaffold for SDF1 Delivery Improves Cardiac Function in a Rat Subacute Myocardial Infarct Model Perez-Estenaga, Iñigo Chevalier, Merari Tumin Peña, Estefania Abizanda, Gloria Alsharabasy, Amir M. Larequi, Eduardo Cilla, Myriam Perez, Marta M. Gurtubay, Jon Garcia-Yebenes Castro, Manuel Prosper, Felipe Pandit, Abhay Pelacho, Beatriz ACS Appl Mater Interfaces [Image: see text] Ischemic heart disease is one of the leading causes of death worldwide. The efficient delivery of therapeutic growth factors could counteract the adverse prognosis of post-myocardial infarction (post-MI). In this study, a collagen hydrogel that is able to load and appropriately deliver pro-angiogenic stromal cell-derived factor 1 (SDF1) was physically coupled with a compact collagen membrane in order to provide the suture strength required for surgical implantation. This bilayer collagen-on-collagen scaffold (bCS) showed the suitable physicochemical properties that are needed for efficient implantation, and the scaffold was able to deliver therapeutic growth factors after MI. In vitro collagen matrix biodegradation led to a sustained SDF1 release and a lack of cytotoxicity in the relevant cell cultures. In vivo intervention in a rat subacute MI model resulted in the full integration of the scaffold into the heart after implantation and biocompatibility with the tissue, with a prevalence of anti-inflammatory and pro-angiogenic macrophages, as well as evidence of revascularization and improved cardiac function after 60 days. Moreover, the beneficial effect of the released SDF1 on heart remodeling was confirmed by a significant reduction in cardiac tissue stiffness. Our findings demonstrate that this multimodal scaffold is a desirable matrix that can be used as a drug delivery system and a scaffolding material to promote functional recovery after MI. American Chemical Society 2023-08-11 /pmc/articles/PMC10636708/ /pubmed/37566441 http://dx.doi.org/10.1021/acsami.3c04245 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Perez-Estenaga, Iñigo Chevalier, Merari Tumin Peña, Estefania Abizanda, Gloria Alsharabasy, Amir M. Larequi, Eduardo Cilla, Myriam Perez, Marta M. Gurtubay, Jon Garcia-Yebenes Castro, Manuel Prosper, Felipe Pandit, Abhay Pelacho, Beatriz A Multimodal Scaffold for SDF1 Delivery Improves Cardiac Function in a Rat Subacute Myocardial Infarct Model |
title | A
Multimodal Scaffold
for SDF1 Delivery Improves Cardiac
Function in a Rat Subacute Myocardial Infarct Model |
title_full | A
Multimodal Scaffold
for SDF1 Delivery Improves Cardiac
Function in a Rat Subacute Myocardial Infarct Model |
title_fullStr | A
Multimodal Scaffold
for SDF1 Delivery Improves Cardiac
Function in a Rat Subacute Myocardial Infarct Model |
title_full_unstemmed | A
Multimodal Scaffold
for SDF1 Delivery Improves Cardiac
Function in a Rat Subacute Myocardial Infarct Model |
title_short | A
Multimodal Scaffold
for SDF1 Delivery Improves Cardiac
Function in a Rat Subacute Myocardial Infarct Model |
title_sort | a
multimodal scaffold
for sdf1 delivery improves cardiac
function in a rat subacute myocardial infarct model |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636708/ https://www.ncbi.nlm.nih.gov/pubmed/37566441 http://dx.doi.org/10.1021/acsami.3c04245 |
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