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Human O-GlcNAcase Uses a Preactivated Boat-skew Substrate Conformation for Catalysis. Evidence from X-ray Crystallography and QM/MM Metadynamics

[Image: see text] Human O-linked β-N-acetylglucosaminidase (hOGA) is one of the two enzymes involved in nuclear and cytoplasmic protein O-GlcNAcylation, an essential post-translational modification. The enzyme catalyzes the hydrolysis of the GlcNAc-O-(Ser/Thr) glycosidic bonds via anchimeric assista...

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Detalles Bibliográficos
Autores principales:	Calvelo, Martín, Males, Alexandra, Alteen, Matthew G., Willems, Lianne I., Vocadlo, David J., Davies, Gideon J., Rovira, Carme
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636738/ https://www.ncbi.nlm.nih.gov/pubmed/37969138 http://dx.doi.org/10.1021/acscatal.3c02378

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