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IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages
Insulin growth factor-1 (IGF-1) is an endocrine regulator that plays an important role in normal growth and development. IGF-1 mediated effects may result in protecting macrophages from immunometabolic response. However, it is unclear whether IGF-1 has a protective effect on fatty acid-induced macro...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636768/ https://www.ncbi.nlm.nih.gov/pubmed/37921069 http://dx.doi.org/10.3892/mmr.2023.13121 |
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author | Tang, Wanying Zhang, Ming Wang, Yu Ma, Dan Hu, Mi Zhang, Yangkai Lin, Huiling Jiang, Weiwei Ouyang, Yuxin Jiang, Liping He, Pingping Zhao, Guojun Ouyang, Xinping |
author_facet | Tang, Wanying Zhang, Ming Wang, Yu Ma, Dan Hu, Mi Zhang, Yangkai Lin, Huiling Jiang, Weiwei Ouyang, Yuxin Jiang, Liping He, Pingping Zhao, Guojun Ouyang, Xinping |
author_sort | Tang, Wanying |
collection | PubMed |
description | Insulin growth factor-1 (IGF-1) is an endocrine regulator that plays an important role in normal growth and development. IGF-1 mediated effects may result in protecting macrophages from immunometabolic response. However, it is unclear whether IGF-1 has a protective effect on fatty acid-induced macrophages damage. In the present study, THP-1 cells were differentiated into macrophages and stimulated with palmitic acid (PA) in the absence or presence of IGF-1. Macrophages apoptosis was measured by Cell Counting Kit-8 assay, flow cytometry, Hoechst 33342 staining and western blotting. The mitochondrial damage was evaluated using JC-1 staining and mitochondrial reactive oxygen species detection. The activation of mitophagy was assessed using immunofluorescence and western blotting. As a result, IGF-1 significantly restored the survival rate in macrophages, while the apoptosis was inhibited through mitochondrial pathway. In addition, IGF-1 protected the mitochondrial damage induced by PA. Furthermore, PA induced mitophagy via phosphatase and tensin homolog-induced putative kinase protein 1/Parkin, which was reversed by IGF-1. Taken together, the present study demonstrated the protective effect of IGF-1 on PA-induced mitochondrial apoptosis in macrophages, which might provide a potential therapeutic strategy for treatment of lipotoxicity. |
format | Online Article Text |
id | pubmed-10636768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-106367682023-11-11 IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages Tang, Wanying Zhang, Ming Wang, Yu Ma, Dan Hu, Mi Zhang, Yangkai Lin, Huiling Jiang, Weiwei Ouyang, Yuxin Jiang, Liping He, Pingping Zhao, Guojun Ouyang, Xinping Mol Med Rep Articles Insulin growth factor-1 (IGF-1) is an endocrine regulator that plays an important role in normal growth and development. IGF-1 mediated effects may result in protecting macrophages from immunometabolic response. However, it is unclear whether IGF-1 has a protective effect on fatty acid-induced macrophages damage. In the present study, THP-1 cells were differentiated into macrophages and stimulated with palmitic acid (PA) in the absence or presence of IGF-1. Macrophages apoptosis was measured by Cell Counting Kit-8 assay, flow cytometry, Hoechst 33342 staining and western blotting. The mitochondrial damage was evaluated using JC-1 staining and mitochondrial reactive oxygen species detection. The activation of mitophagy was assessed using immunofluorescence and western blotting. As a result, IGF-1 significantly restored the survival rate in macrophages, while the apoptosis was inhibited through mitochondrial pathway. In addition, IGF-1 protected the mitochondrial damage induced by PA. Furthermore, PA induced mitophagy via phosphatase and tensin homolog-induced putative kinase protein 1/Parkin, which was reversed by IGF-1. Taken together, the present study demonstrated the protective effect of IGF-1 on PA-induced mitochondrial apoptosis in macrophages, which might provide a potential therapeutic strategy for treatment of lipotoxicity. D.A. Spandidos 2023-11-01 /pmc/articles/PMC10636768/ /pubmed/37921069 http://dx.doi.org/10.3892/mmr.2023.13121 Text en Copyright: © Tang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Tang, Wanying Zhang, Ming Wang, Yu Ma, Dan Hu, Mi Zhang, Yangkai Lin, Huiling Jiang, Weiwei Ouyang, Yuxin Jiang, Liping He, Pingping Zhao, Guojun Ouyang, Xinping IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages |
title | IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages |
title_full | IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages |
title_fullStr | IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages |
title_full_unstemmed | IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages |
title_short | IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages |
title_sort | igf‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636768/ https://www.ncbi.nlm.nih.gov/pubmed/37921069 http://dx.doi.org/10.3892/mmr.2023.13121 |
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