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Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope

BACKGROUND: Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered a subset of domain-inlaid Nme2Cas9 ba...

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Detalles Bibliográficos
Autores principales:	Zhao, Ding, Gao, Xun, Zhou, Jiale, Li, Jinze, Qian, Yuqiang, Wang, Di, Niu, Wenchao, Zhang, Tao, Hu, Mingyang, Xiong, Haoyang, Lai, Liangxue, Li, Zhanjun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636962/ https://www.ncbi.nlm.nih.gov/pubmed/37946200 http://dx.doi.org/10.1186/s12915-023-01754-4

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