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Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization

Cerebral ischemia/reperfusion (CI/R) injury is a clinical conundrum during the treatment of ischemic stroke. Cell-derived exosomes (CDE) were proved to be therapeutically effective for CI/R injury. However, production of CDE is time and effort consuming. Increasing studies reported that plants can a...

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Autores principales: Li, Shiyi, Zhang, Ru, Wang, Anni, Li, Yang, Zhang, Miaomiao, Kim, Jisu, Zhu, Ying, Wang, Qizheng, Zhang, Yue, Wei, Ying, Wang, Jianxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636993/
https://www.ncbi.nlm.nih.gov/pubmed/37946257
http://dx.doi.org/10.1186/s12951-023-02161-1
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author Li, Shiyi
Zhang, Ru
Wang, Anni
Li, Yang
Zhang, Miaomiao
Kim, Jisu
Zhu, Ying
Wang, Qizheng
Zhang, Yue
Wei, Ying
Wang, Jianxin
author_facet Li, Shiyi
Zhang, Ru
Wang, Anni
Li, Yang
Zhang, Miaomiao
Kim, Jisu
Zhu, Ying
Wang, Qizheng
Zhang, Yue
Wei, Ying
Wang, Jianxin
author_sort Li, Shiyi
collection PubMed
description Cerebral ischemia/reperfusion (CI/R) injury is a clinical conundrum during the treatment of ischemic stroke. Cell-derived exosomes (CDE) were proved to be therapeutically effective for CI/R injury. However, production of CDE is time and effort consuming. Increasing studies reported that plants can also generate exosome-like nanoparticles (ELN) which are therapeutically effective and have higher yield compared with CDE. In this study, a commonly used Chinese herb Panax notoginseng (PN), whose active ingredients were well-documented in the treatment of CI/R injury, was chosen as a source of ELNs. It was found that Panax notoginseng derived exosome like nanoparticles (PDN) could enter the brain without modification and ameliorate cerebral infarct volume, improve behavior outcome and maintained the integrity of BBB. PDNs attenuated CI/R injury by altering the phenotype of microglia from “pro-inflammation” M1 type to “anti-inflammation” M2 type. Also, we found that lipids from PDNs were the major therapeutic effective component. As a mechanism of action, PDN was proved to exert therapeutic effect via activating pI3k/Akt pathway. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-023-02161-1.
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spelling pubmed-106369932023-11-11 Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization Li, Shiyi Zhang, Ru Wang, Anni Li, Yang Zhang, Miaomiao Kim, Jisu Zhu, Ying Wang, Qizheng Zhang, Yue Wei, Ying Wang, Jianxin J Nanobiotechnology Research Cerebral ischemia/reperfusion (CI/R) injury is a clinical conundrum during the treatment of ischemic stroke. Cell-derived exosomes (CDE) were proved to be therapeutically effective for CI/R injury. However, production of CDE is time and effort consuming. Increasing studies reported that plants can also generate exosome-like nanoparticles (ELN) which are therapeutically effective and have higher yield compared with CDE. In this study, a commonly used Chinese herb Panax notoginseng (PN), whose active ingredients were well-documented in the treatment of CI/R injury, was chosen as a source of ELNs. It was found that Panax notoginseng derived exosome like nanoparticles (PDN) could enter the brain without modification and ameliorate cerebral infarct volume, improve behavior outcome and maintained the integrity of BBB. PDNs attenuated CI/R injury by altering the phenotype of microglia from “pro-inflammation” M1 type to “anti-inflammation” M2 type. Also, we found that lipids from PDNs were the major therapeutic effective component. As a mechanism of action, PDN was proved to exert therapeutic effect via activating pI3k/Akt pathway. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-023-02161-1. BioMed Central 2023-11-10 /pmc/articles/PMC10636993/ /pubmed/37946257 http://dx.doi.org/10.1186/s12951-023-02161-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Shiyi
Zhang, Ru
Wang, Anni
Li, Yang
Zhang, Miaomiao
Kim, Jisu
Zhu, Ying
Wang, Qizheng
Zhang, Yue
Wei, Ying
Wang, Jianxin
Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
title Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
title_full Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
title_fullStr Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
title_full_unstemmed Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
title_short Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
title_sort panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636993/
https://www.ncbi.nlm.nih.gov/pubmed/37946257
http://dx.doi.org/10.1186/s12951-023-02161-1
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