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The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods

BACKGROUND: Microbiota composition is fundamental to human health with the intestinal microbiota undergoing critical changes within the first two years of life. The developing intestinal microbiota is shaped by maternal seeding, breast milk and its complex constituents, other nutrients, and the envi...

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Autores principales: Cecchini, Luca, Barmaz, Colette, Cea, Maria José Coloma, Baeschlin, Hannah, Etter, Julian, Netzer, Stefanie, Bregy, Leonie, Marchukov, Dmitrij, Trigo, Nerea Fernandez, Meier, Rachel, Hirschi, Jasmin, Wyss, Jacqueline, Wick, Andrina, Zingg, Joelle, Christensen, Sandro, Radan, Anda-Petronela, Etter, Annina, Müller, Martin, Kaess, Michael, Surbek, Daniel, Yilmaz, Bahtiyar, Macpherson, Andrew J., Sokollik, Christiane, Misselwitz, Benjamin, Ganal-Vonarburg, Stephanie C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637001/
https://www.ncbi.nlm.nih.gov/pubmed/37946167
http://dx.doi.org/10.1186/s12887-023-04198-5
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author Cecchini, Luca
Barmaz, Colette
Cea, Maria José Coloma
Baeschlin, Hannah
Etter, Julian
Netzer, Stefanie
Bregy, Leonie
Marchukov, Dmitrij
Trigo, Nerea Fernandez
Meier, Rachel
Hirschi, Jasmin
Wyss, Jacqueline
Wick, Andrina
Zingg, Joelle
Christensen, Sandro
Radan, Anda-Petronela
Etter, Annina
Müller, Martin
Kaess, Michael
Surbek, Daniel
Yilmaz, Bahtiyar
Macpherson, Andrew J.
Sokollik, Christiane
Misselwitz, Benjamin
Ganal-Vonarburg, Stephanie C.
author_facet Cecchini, Luca
Barmaz, Colette
Cea, Maria José Coloma
Baeschlin, Hannah
Etter, Julian
Netzer, Stefanie
Bregy, Leonie
Marchukov, Dmitrij
Trigo, Nerea Fernandez
Meier, Rachel
Hirschi, Jasmin
Wyss, Jacqueline
Wick, Andrina
Zingg, Joelle
Christensen, Sandro
Radan, Anda-Petronela
Etter, Annina
Müller, Martin
Kaess, Michael
Surbek, Daniel
Yilmaz, Bahtiyar
Macpherson, Andrew J.
Sokollik, Christiane
Misselwitz, Benjamin
Ganal-Vonarburg, Stephanie C.
author_sort Cecchini, Luca
collection PubMed
description BACKGROUND: Microbiota composition is fundamental to human health with the intestinal microbiota undergoing critical changes within the first two years of life. The developing intestinal microbiota is shaped by maternal seeding, breast milk and its complex constituents, other nutrients, and the environment. Understanding microbiota-dependent pathologies requires a profound understanding of the early development of the healthy infant microbiota. METHODS: Two hundred and fifty healthy pregnant women (≥20 weeks of gestation) from the greater Bern area will be enrolled at Bern University hospital’s maternity department. Participants will be followed as mother-baby pairs at delivery, week(s) 1, 2, 6, 10, 14, 24, 36, 48, 96, and at years 5 and 10 after birth. Clinical parameters describing infant growth and development, morbidity, and allergic conditions as well as socio-economic, nutritional, and epidemiological data will be documented. Neuro-developmental outcomes and behavior will be assessed by child behavior checklists at and beyond 2 years of age. Maternal stool, milk, skin and vaginal swabs, infant stool, and skin swabs will be collected at enrolment and at follow-up visits. For the primary outcome, the trajectory of the infant intestinal microbiota will be characterized by 16S and metagenomic sequencing regarding composition, metabolic potential, and stability during the first 2 years of life. Secondary outcomes will assess the cellular and chemical composition of maternal milk, the impact of nutrition and environment on microbiota development, the maternal microbiome transfer at vaginal or caesarean birth and thereafter on the infant, and correlate parameters of microbiota and maternal milk on infant growth, development, health, and mental well-being. DISCUSSION: The Bern birth cohort study will provide a detailed description and normal ranges of the trajectory of microbiota maturation in a high-resource setting. These data will be compared to data from low-resource settings such as from the Zimbabwe-College of Health-Sciences-Birth-Cohort study. Prospective bio-sampling and data collection will allow studying the association of the microbiota with common childhood conditions concerning allergies, obesity, neuro-developmental outcomes , and behaviour. Trial registration The trial has been registered at www.clinicaltrials.gov, Identifier: NCT04447742
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spelling pubmed-106370012023-11-11 The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods Cecchini, Luca Barmaz, Colette Cea, Maria José Coloma Baeschlin, Hannah Etter, Julian Netzer, Stefanie Bregy, Leonie Marchukov, Dmitrij Trigo, Nerea Fernandez Meier, Rachel Hirschi, Jasmin Wyss, Jacqueline Wick, Andrina Zingg, Joelle Christensen, Sandro Radan, Anda-Petronela Etter, Annina Müller, Martin Kaess, Michael Surbek, Daniel Yilmaz, Bahtiyar Macpherson, Andrew J. Sokollik, Christiane Misselwitz, Benjamin Ganal-Vonarburg, Stephanie C. BMC Pediatr Study Protocol BACKGROUND: Microbiota composition is fundamental to human health with the intestinal microbiota undergoing critical changes within the first two years of life. The developing intestinal microbiota is shaped by maternal seeding, breast milk and its complex constituents, other nutrients, and the environment. Understanding microbiota-dependent pathologies requires a profound understanding of the early development of the healthy infant microbiota. METHODS: Two hundred and fifty healthy pregnant women (≥20 weeks of gestation) from the greater Bern area will be enrolled at Bern University hospital’s maternity department. Participants will be followed as mother-baby pairs at delivery, week(s) 1, 2, 6, 10, 14, 24, 36, 48, 96, and at years 5 and 10 after birth. Clinical parameters describing infant growth and development, morbidity, and allergic conditions as well as socio-economic, nutritional, and epidemiological data will be documented. Neuro-developmental outcomes and behavior will be assessed by child behavior checklists at and beyond 2 years of age. Maternal stool, milk, skin and vaginal swabs, infant stool, and skin swabs will be collected at enrolment and at follow-up visits. For the primary outcome, the trajectory of the infant intestinal microbiota will be characterized by 16S and metagenomic sequencing regarding composition, metabolic potential, and stability during the first 2 years of life. Secondary outcomes will assess the cellular and chemical composition of maternal milk, the impact of nutrition and environment on microbiota development, the maternal microbiome transfer at vaginal or caesarean birth and thereafter on the infant, and correlate parameters of microbiota and maternal milk on infant growth, development, health, and mental well-being. DISCUSSION: The Bern birth cohort study will provide a detailed description and normal ranges of the trajectory of microbiota maturation in a high-resource setting. These data will be compared to data from low-resource settings such as from the Zimbabwe-College of Health-Sciences-Birth-Cohort study. Prospective bio-sampling and data collection will allow studying the association of the microbiota with common childhood conditions concerning allergies, obesity, neuro-developmental outcomes , and behaviour. Trial registration The trial has been registered at www.clinicaltrials.gov, Identifier: NCT04447742 BioMed Central 2023-11-10 /pmc/articles/PMC10637001/ /pubmed/37946167 http://dx.doi.org/10.1186/s12887-023-04198-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Study Protocol
Cecchini, Luca
Barmaz, Colette
Cea, Maria José Coloma
Baeschlin, Hannah
Etter, Julian
Netzer, Stefanie
Bregy, Leonie
Marchukov, Dmitrij
Trigo, Nerea Fernandez
Meier, Rachel
Hirschi, Jasmin
Wyss, Jacqueline
Wick, Andrina
Zingg, Joelle
Christensen, Sandro
Radan, Anda-Petronela
Etter, Annina
Müller, Martin
Kaess, Michael
Surbek, Daniel
Yilmaz, Bahtiyar
Macpherson, Andrew J.
Sokollik, Christiane
Misselwitz, Benjamin
Ganal-Vonarburg, Stephanie C.
The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods
title The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods
title_full The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods
title_fullStr The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods
title_full_unstemmed The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods
title_short The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods
title_sort bern birth cohort (bebico) to study the development of the infant intestinal microbiota in a high-resource setting in switzerland: rationale, design, and methods
topic Study Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637001/
https://www.ncbi.nlm.nih.gov/pubmed/37946167
http://dx.doi.org/10.1186/s12887-023-04198-5
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