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The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods
BACKGROUND: Microbiota composition is fundamental to human health with the intestinal microbiota undergoing critical changes within the first two years of life. The developing intestinal microbiota is shaped by maternal seeding, breast milk and its complex constituents, other nutrients, and the envi...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637001/ https://www.ncbi.nlm.nih.gov/pubmed/37946167 http://dx.doi.org/10.1186/s12887-023-04198-5 |
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author | Cecchini, Luca Barmaz, Colette Cea, Maria José Coloma Baeschlin, Hannah Etter, Julian Netzer, Stefanie Bregy, Leonie Marchukov, Dmitrij Trigo, Nerea Fernandez Meier, Rachel Hirschi, Jasmin Wyss, Jacqueline Wick, Andrina Zingg, Joelle Christensen, Sandro Radan, Anda-Petronela Etter, Annina Müller, Martin Kaess, Michael Surbek, Daniel Yilmaz, Bahtiyar Macpherson, Andrew J. Sokollik, Christiane Misselwitz, Benjamin Ganal-Vonarburg, Stephanie C. |
author_facet | Cecchini, Luca Barmaz, Colette Cea, Maria José Coloma Baeschlin, Hannah Etter, Julian Netzer, Stefanie Bregy, Leonie Marchukov, Dmitrij Trigo, Nerea Fernandez Meier, Rachel Hirschi, Jasmin Wyss, Jacqueline Wick, Andrina Zingg, Joelle Christensen, Sandro Radan, Anda-Petronela Etter, Annina Müller, Martin Kaess, Michael Surbek, Daniel Yilmaz, Bahtiyar Macpherson, Andrew J. Sokollik, Christiane Misselwitz, Benjamin Ganal-Vonarburg, Stephanie C. |
author_sort | Cecchini, Luca |
collection | PubMed |
description | BACKGROUND: Microbiota composition is fundamental to human health with the intestinal microbiota undergoing critical changes within the first two years of life. The developing intestinal microbiota is shaped by maternal seeding, breast milk and its complex constituents, other nutrients, and the environment. Understanding microbiota-dependent pathologies requires a profound understanding of the early development of the healthy infant microbiota. METHODS: Two hundred and fifty healthy pregnant women (≥20 weeks of gestation) from the greater Bern area will be enrolled at Bern University hospital’s maternity department. Participants will be followed as mother-baby pairs at delivery, week(s) 1, 2, 6, 10, 14, 24, 36, 48, 96, and at years 5 and 10 after birth. Clinical parameters describing infant growth and development, morbidity, and allergic conditions as well as socio-economic, nutritional, and epidemiological data will be documented. Neuro-developmental outcomes and behavior will be assessed by child behavior checklists at and beyond 2 years of age. Maternal stool, milk, skin and vaginal swabs, infant stool, and skin swabs will be collected at enrolment and at follow-up visits. For the primary outcome, the trajectory of the infant intestinal microbiota will be characterized by 16S and metagenomic sequencing regarding composition, metabolic potential, and stability during the first 2 years of life. Secondary outcomes will assess the cellular and chemical composition of maternal milk, the impact of nutrition and environment on microbiota development, the maternal microbiome transfer at vaginal or caesarean birth and thereafter on the infant, and correlate parameters of microbiota and maternal milk on infant growth, development, health, and mental well-being. DISCUSSION: The Bern birth cohort study will provide a detailed description and normal ranges of the trajectory of microbiota maturation in a high-resource setting. These data will be compared to data from low-resource settings such as from the Zimbabwe-College of Health-Sciences-Birth-Cohort study. Prospective bio-sampling and data collection will allow studying the association of the microbiota with common childhood conditions concerning allergies, obesity, neuro-developmental outcomes , and behaviour. Trial registration The trial has been registered at www.clinicaltrials.gov, Identifier: NCT04447742 |
format | Online Article Text |
id | pubmed-10637001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-106370012023-11-11 The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods Cecchini, Luca Barmaz, Colette Cea, Maria José Coloma Baeschlin, Hannah Etter, Julian Netzer, Stefanie Bregy, Leonie Marchukov, Dmitrij Trigo, Nerea Fernandez Meier, Rachel Hirschi, Jasmin Wyss, Jacqueline Wick, Andrina Zingg, Joelle Christensen, Sandro Radan, Anda-Petronela Etter, Annina Müller, Martin Kaess, Michael Surbek, Daniel Yilmaz, Bahtiyar Macpherson, Andrew J. Sokollik, Christiane Misselwitz, Benjamin Ganal-Vonarburg, Stephanie C. BMC Pediatr Study Protocol BACKGROUND: Microbiota composition is fundamental to human health with the intestinal microbiota undergoing critical changes within the first two years of life. The developing intestinal microbiota is shaped by maternal seeding, breast milk and its complex constituents, other nutrients, and the environment. Understanding microbiota-dependent pathologies requires a profound understanding of the early development of the healthy infant microbiota. METHODS: Two hundred and fifty healthy pregnant women (≥20 weeks of gestation) from the greater Bern area will be enrolled at Bern University hospital’s maternity department. Participants will be followed as mother-baby pairs at delivery, week(s) 1, 2, 6, 10, 14, 24, 36, 48, 96, and at years 5 and 10 after birth. Clinical parameters describing infant growth and development, morbidity, and allergic conditions as well as socio-economic, nutritional, and epidemiological data will be documented. Neuro-developmental outcomes and behavior will be assessed by child behavior checklists at and beyond 2 years of age. Maternal stool, milk, skin and vaginal swabs, infant stool, and skin swabs will be collected at enrolment and at follow-up visits. For the primary outcome, the trajectory of the infant intestinal microbiota will be characterized by 16S and metagenomic sequencing regarding composition, metabolic potential, and stability during the first 2 years of life. Secondary outcomes will assess the cellular and chemical composition of maternal milk, the impact of nutrition and environment on microbiota development, the maternal microbiome transfer at vaginal or caesarean birth and thereafter on the infant, and correlate parameters of microbiota and maternal milk on infant growth, development, health, and mental well-being. DISCUSSION: The Bern birth cohort study will provide a detailed description and normal ranges of the trajectory of microbiota maturation in a high-resource setting. These data will be compared to data from low-resource settings such as from the Zimbabwe-College of Health-Sciences-Birth-Cohort study. Prospective bio-sampling and data collection will allow studying the association of the microbiota with common childhood conditions concerning allergies, obesity, neuro-developmental outcomes , and behaviour. Trial registration The trial has been registered at www.clinicaltrials.gov, Identifier: NCT04447742 BioMed Central 2023-11-10 /pmc/articles/PMC10637001/ /pubmed/37946167 http://dx.doi.org/10.1186/s12887-023-04198-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Study Protocol Cecchini, Luca Barmaz, Colette Cea, Maria José Coloma Baeschlin, Hannah Etter, Julian Netzer, Stefanie Bregy, Leonie Marchukov, Dmitrij Trigo, Nerea Fernandez Meier, Rachel Hirschi, Jasmin Wyss, Jacqueline Wick, Andrina Zingg, Joelle Christensen, Sandro Radan, Anda-Petronela Etter, Annina Müller, Martin Kaess, Michael Surbek, Daniel Yilmaz, Bahtiyar Macpherson, Andrew J. Sokollik, Christiane Misselwitz, Benjamin Ganal-Vonarburg, Stephanie C. The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods |
title | The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods |
title_full | The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods |
title_fullStr | The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods |
title_full_unstemmed | The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods |
title_short | The Bern Birth Cohort (BeBiCo) to study the development of the infant intestinal microbiota in a high-resource setting in Switzerland: rationale, design, and methods |
title_sort | bern birth cohort (bebico) to study the development of the infant intestinal microbiota in a high-resource setting in switzerland: rationale, design, and methods |
topic | Study Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637001/ https://www.ncbi.nlm.nih.gov/pubmed/37946167 http://dx.doi.org/10.1186/s12887-023-04198-5 |
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