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Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment
Expression of the gene for collagen XVII (COL17A1) in tumor tissue is positively or negatively associated with patient survival depending on cancer type. High COL17A1 expression is thus a favorable prognostic marker for breast cancer but unfavorable for pancreatic cancer. This study explored the eff...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637054/ https://www.ncbi.nlm.nih.gov/pubmed/37688308 http://dx.doi.org/10.1111/cas.15952 |
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author | Kashiwagi, Ryosuke Funayama, Ryo Aoki, Shuichi Matsui, Aya Klein, Sebastian Sato, Yukihiro Suzuki, Tsubasa Murakami, Keigo Inoue, Koetsu Iseki, Masahiro Masuda, Kunihiro Mizuma, Masamichi Naito, Hisamichi Duda, Dan G. Unno, Michiaki Nakayama, Keiko |
author_facet | Kashiwagi, Ryosuke Funayama, Ryo Aoki, Shuichi Matsui, Aya Klein, Sebastian Sato, Yukihiro Suzuki, Tsubasa Murakami, Keigo Inoue, Koetsu Iseki, Masahiro Masuda, Kunihiro Mizuma, Masamichi Naito, Hisamichi Duda, Dan G. Unno, Michiaki Nakayama, Keiko |
author_sort | Kashiwagi, Ryosuke |
collection | PubMed |
description | Expression of the gene for collagen XVII (COL17A1) in tumor tissue is positively or negatively associated with patient survival depending on cancer type. High COL17A1 expression is thus a favorable prognostic marker for breast cancer but unfavorable for pancreatic cancer. This study explored the effects of COL17A1 expression on pancreatic tumor growth and their underlying mechanisms. Analysis of published single‐cell RNA‐sequencing data for human pancreatic cancer tissue revealed that COL17A1 was expressed predominantly in cancer cells rather than surrounding stromal cells. Forced expression of COL17A1 did not substantially affect the proliferation rate of the mouse pancreatic cancer cell lines KPC and AK4.4 in vitro. However, in mouse homograft tumor models in which KPC or AK4.4 cells were injected into syngeneic C57BL/6 or FVB mice, respectively, COL17A1 expression promoted or suppressed tumor growth, respectively, suggesting that the effect of COL17A1 on tumor growth was influenced by the tumor microenvironment. RNA‐sequencing analysis of tumor tissue revealed effects of COL17A1 on gene expression profiles (including the expression of genes related to cell proliferation, the immune response, Wnt signaling, and Hippo signaling) that differed between C57BL/6‐KPC and FVB‐AK4.4 tumors. Our data thus suggest that COL17A1 promotes or suppresses cancer progression in a manner dependent on the interaction of tumor cells with the tumor microenvironment. |
format | Online Article Text |
id | pubmed-10637054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106370542023-11-15 Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment Kashiwagi, Ryosuke Funayama, Ryo Aoki, Shuichi Matsui, Aya Klein, Sebastian Sato, Yukihiro Suzuki, Tsubasa Murakami, Keigo Inoue, Koetsu Iseki, Masahiro Masuda, Kunihiro Mizuma, Masamichi Naito, Hisamichi Duda, Dan G. Unno, Michiaki Nakayama, Keiko Cancer Sci Original Articles Expression of the gene for collagen XVII (COL17A1) in tumor tissue is positively or negatively associated with patient survival depending on cancer type. High COL17A1 expression is thus a favorable prognostic marker for breast cancer but unfavorable for pancreatic cancer. This study explored the effects of COL17A1 expression on pancreatic tumor growth and their underlying mechanisms. Analysis of published single‐cell RNA‐sequencing data for human pancreatic cancer tissue revealed that COL17A1 was expressed predominantly in cancer cells rather than surrounding stromal cells. Forced expression of COL17A1 did not substantially affect the proliferation rate of the mouse pancreatic cancer cell lines KPC and AK4.4 in vitro. However, in mouse homograft tumor models in which KPC or AK4.4 cells were injected into syngeneic C57BL/6 or FVB mice, respectively, COL17A1 expression promoted or suppressed tumor growth, respectively, suggesting that the effect of COL17A1 on tumor growth was influenced by the tumor microenvironment. RNA‐sequencing analysis of tumor tissue revealed effects of COL17A1 on gene expression profiles (including the expression of genes related to cell proliferation, the immune response, Wnt signaling, and Hippo signaling) that differed between C57BL/6‐KPC and FVB‐AK4.4 tumors. Our data thus suggest that COL17A1 promotes or suppresses cancer progression in a manner dependent on the interaction of tumor cells with the tumor microenvironment. John Wiley and Sons Inc. 2023-09-08 /pmc/articles/PMC10637054/ /pubmed/37688308 http://dx.doi.org/10.1111/cas.15952 Text en © 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Kashiwagi, Ryosuke Funayama, Ryo Aoki, Shuichi Matsui, Aya Klein, Sebastian Sato, Yukihiro Suzuki, Tsubasa Murakami, Keigo Inoue, Koetsu Iseki, Masahiro Masuda, Kunihiro Mizuma, Masamichi Naito, Hisamichi Duda, Dan G. Unno, Michiaki Nakayama, Keiko Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment |
title | Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment |
title_full | Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment |
title_fullStr | Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment |
title_full_unstemmed | Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment |
title_short | Collagen XVII regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment |
title_sort | collagen xvii regulates tumor growth in pancreatic cancer through interaction with the tumor microenvironment |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637054/ https://www.ncbi.nlm.nih.gov/pubmed/37688308 http://dx.doi.org/10.1111/cas.15952 |
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